Cultivo de tejidos
Material type: ArticleLanguage: Spanish Analytics: Show analyticsDescription: p. 115-125Subject(s):- Manihot esculenta
- Apical meristems
- Cassava mosaic virus
- CASSAVA PROGRAMS
- Colombia
- Development
- Disease control
- Germplasm
- Pest control
- South America
- Soybean flour
- Tissue culture
- America
- DISEASES AND PATHOGENS
- Flours
- Pests
- Plant tissues
- Viroses
- Manihot esculenta
- Meristemas apicales
- Virus de mosaico de yuca
- PROGRAMAS DE YUCA
- Colombia
- Desarrollo
- Control de enfermedades
- Germoplasma
- Control de plagas
- AMERICA DEL SUR
- HARINA DE SOYA
- Cultivo de tejidos
- Yuca
- Chapters in reports
- Capítulos de informes
- CIAT Editor
- Cassava
- Book chapters
- DIGITAL2015
- 18968
Item type | Current library | Collection | Call number | Status | Date due | Barcode | Item holds | |
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Book Chapters | CIAT Library Electronic documents collection | Electronic documents collection | Available | |||||
Books | CIAT Library CIAT Publications | CIAT Publications | 18968 (Browse shelf(Opens below)) | Available |
During 1981, the efforts to use meristem-culture methods have continued, together with thermotherapy for recovering healthy clones. Frog skin disease was eradicated up to 100 percent in different cassava clones during various propagation cycles through the culture of small meristem tips. A routine "cleaning-up" program consists of: (1) disinfestation of stakes containing dormant buds and treatment with thermotherapy; (2) cutting and disinfestation of shoot terminal buds; (3) isolation of meristem tips (0.5-0.6 mm) under sterile conditions and planting in culture medium; (4) transfer of meristem tips and nodes grown from the meristem to a rooting medium; (5) hardening of rooted plantlets prior to potting; (6) transplant to the field and to the greenhouse; (7) evaluation of the presence of frog skin disease after 3 mo. of growth; and (8) selection of symptom-free plants, grown again for 3 mo., and reevaluated. A germplasm bank in vitro is a long-term goal to conserve material and to isolate microorganisms. The main steps and procedures followed in the international exchange of clonal materials are described. During 1981, work was initiated aimed atdeveloping anther-culture methods for cassava and to observe microsporogenesis stages in relation to the size of the floral bud. (CIAT)