Development of methodologies for the isolation and culture of cassava immature pollen and zygotic embryos

By:

Cataño C., Martha Lucía

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Material type: Article

ArticleLanguage: English Description: p. 185-189Subject(s): LOC classification:

SB 211 .C3 I57

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In: In: Roca, William M.; Thro, Ann Marie (eds.). International Scientific Meeting Cassava Biotechnology Network (1, 1992, Cartagena de Indias, Colombia). ProceedingsSummary: Highly efficient systems for the isolation and in vitro culture of cassava pollen and zygotic embryos have been developed. These methodologies have allowed the manipulation of large quantities of isolated pollen from immature and mature anthers for use in studies towards haploid induction and pollen cryopreservation. Conditions were also developed for the isolation, in vitro culture and germination of cassava immature zygotic embryos. The technique was used to recover interspecific Manihot hybrid populations for use in the construction of a cassava molecular linkage map. Culture media and conditions for isolation and culture of pollen and zygotic embryos, and results of work carried out towards the induction of haploidy, will be presented.

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Item type	Current library	Collection	Call number	Status	Date due	Barcode	Item holds
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Books	CIAT Library CIAT Publications	CIAT Publications	SB 211 .C3 I57 (Browse shelf(Opens below))	Available

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Highly efficient systems for the isolation and in vitro culture of cassava pollen and zygotic embryos have been developed. These methodologies have allowed the manipulation of large quantities of isolated pollen from immature and mature anthers for use in studies towards haploid induction and pollen cryopreservation. Conditions were also developed for the isolation, in vitro culture and germination of cassava immature zygotic embryos. The technique was used to recover interspecific Manihot hybrid populations for use in the construction of a cassava molecular linkage map. Culture media and conditions for isolation and culture of pollen and zygotic embryos, and results of work carried out towards the induction of haploidy, will be presented.