Embriogenese somatica a partir de folhas imaturas de mandioca in vitro

Se estudiaron las condiciones para la induccion y el crecimiento de los embriones somaticos en yuca. Se indujeron callos embrionicos friables de hojas inmaduras cuando se cultivaron en un medio Murashige y Skoog modificado que contenia una alta concn. de 2,4-D. Se observaron embriones somaticos cuando los callos fueron transferidos a un medio sin 2,4-D o con baja concn. La induccion de embriones somaticos fue mas eficiente en el medio suplementado con 0.1 mg de 2,4-D/l mas 1 mg de AG/l, seguido de 2 semanas de pre- cultivo en un medio que contiene 10 mg de 2,4-D/l. El no. de embriones somaticos aumento en el medio suplementado con 1 mg de AG/l, sugiriendo que el AG desempeno un papel importante en la induccion y el desarrollo de embriones en yuca. (RA-CIAT)

The conditions for induction and growth of somatic embryos in cassava (Manihot esculenta Crantz) were studied. Friable embryogenic calluses were induced from immature leaves when they were cultured in a modified Murashige and Skoog's medium containing high concentration of 2,4-dichlorophenoxyacetic acid (2,4-D). Somatic embryos were observed when the calluses were transferred to a medium without 2,4-D or with low concentration of it. Somatic embryo induction was more efficient on the medium supplemented with 0.1 mg/l 2,4-D + 1 mg/l gibberelic acid (GA3), followed by 2 weeks of pre-culture on a medium containing 10 mg/l 2,4-D. The number of somatic embryos increased on the medium supplemented with 1 mg/l GA3, suggesting that gibberelic acid plays an important role in embryo induction and development in cassava. (AS)