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Caracterizacion de una nueva proteina como factor responsable de la resistencia de Phaseolus vulgaris a Zabrotes subfasciatus (Boheman) (Coleoptera: Bruchidae)

By: Contributor(s): Material type: ArticleArticleLanguage: Spanish Description: 15(2):3-9Subject(s): LOC classification:
  • 39254
 In: Revista Colombiana de EntomologiaSummary: La Arcelina es una proteina nueva, unicamente presente en frijoles silvestres resistentes a Zabrotes subfasciatus (Boheman), se evaluo como posible factor antibiotico de resistencia a esta plaga. Cuando se introdujo el gen de la proteina en frijoles cultivados susceptibles, mediante un sistema de retrocruzas, se comprobo que la expresion de la proteina esta controlada por un gen mendeliano simple y que su presencia es dominante a su ausencia, pues las lineas homocigotas dominantes resultaron altamente resistentes al insecto. En semillas artificiales, con dosis crecientes de la proteina purificada, se comprobo el efecto antibiotico de la arcelina y se encontro que la concentracion letal media fue 6.5 por ciento. Como la presencia de la arcelina es un marcador de resistencia, se ha implementado una tecnica serologica para detectarla en poblaciones segregantes de frijol. Las semillas positivas (Arc +) se multiplican, para evaluarlas con el insecto, a partir de F3 y generaciones subsiguientes, con el fin de obtener frijoles comerciales resistentes a Z. subfasciatus. (AS)Summary: Arcelin, a new protein, present only in wild beans resistant to Zabrotes subsfasciatus, was evaluated as a possible antibiotic factor of resistance to this pest. When arcelin was introduced in cultivated susceptible beans by means of blackcrosses, it was demonstrated that arcelin expression is controlled by a single mendelian gen and its presence is dominant to its absence. Thus, dominant homocygous lines were highly resistant to the insect, while homocygous recessive lines were highly susceptible. Heterocygous lines were intermediate. The antibiotic effect of arcelin was demonstrated in artificial bean seeds containing increasing levels of the purified protein; the medium lethal concentration (LC50) was 6.5 percent. The presence of arcelin can be used as a marker for resistance to the insect. A serological technique has been implemented to detect the protein in segregating populations of beans. Positive seeds are multiplied and evaluated for reaction to the insect in F3 and following generations. This method is being utilized to obtain commercial beans resistant to Z. subfasciatus. (AS)
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Journal Article Journal Article CIAT Library Document collection CINFOS Document Collection CINFOS 39254 (Browse shelf(Opens below)) c.1 Short Loan 100051516
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La Arcelina es una proteina nueva, unicamente presente en frijoles silvestres resistentes a Zabrotes subfasciatus (Boheman), se evaluo como posible factor antibiotico de resistencia a esta plaga. Cuando se introdujo el gen de la proteina en frijoles cultivados susceptibles, mediante un sistema de retrocruzas, se comprobo que la expresion de la proteina esta controlada por un gen mendeliano simple y que su presencia es dominante a su ausencia, pues las lineas homocigotas dominantes resultaron altamente resistentes al insecto. En semillas artificiales, con dosis crecientes de la proteina purificada, se comprobo el efecto antibiotico de la arcelina y se encontro que la concentracion letal media fue 6.5 por ciento. Como la presencia de la arcelina es un marcador de resistencia, se ha implementado una tecnica serologica para detectarla en poblaciones segregantes de frijol. Las semillas positivas (Arc +) se multiplican, para evaluarlas con el insecto, a partir de F3 y generaciones subsiguientes, con el fin de obtener frijoles comerciales resistentes a Z. subfasciatus. (AS) spa

Arcelin, a new protein, present only in wild beans resistant to Zabrotes subsfasciatus, was evaluated as a possible antibiotic factor of resistance to this pest. When arcelin was introduced in cultivated susceptible beans by means of blackcrosses, it was demonstrated that arcelin expression is controlled by a single mendelian gen and its presence is dominant to its absence. Thus, dominant homocygous lines were highly resistant to the insect, while homocygous recessive lines were highly susceptible. Heterocygous lines were intermediate. The antibiotic effect of arcelin was demonstrated in artificial bean seeds containing increasing levels of the purified protein; the medium lethal concentration (LC50) was 6.5 percent. The presence of arcelin can be used as a marker for resistance to the insect. A serological technique has been implemented to detect the protein in segregating populations of beans. Positive seeds are multiplied and evaluated for reaction to the insect in F3 and following generations. This method is being utilized to obtain commercial beans resistant to Z. subfasciatus. (AS) eng

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