MARC details
| 000 -LEADER |
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| fixed length control field |
02999naa a22004697a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
39649 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
CO-PlCIA |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20130510154429.0 |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
CO-PlCIA |
| Transcribing agency |
CO-PlCIA |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 050 ## - LIBRARY OF CONGRESS CALL NUMBER |
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| Classification number |
41174 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
120502t1987 000 0seng d |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
MEHDY, M.C. |
| 245 ## - TITLE STATEMENT |
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| Title |
Induction of chalcone isomerase gene expression in plant cells in response to fungal cell wall components |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
p. 425-435 |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc |
Plants respond to irradiation by producing protective anthocyanin pigments and to infection by producing phytoalexin antibiotics. Synthesis of anthocyanins and some flavonoid pigments is also developmentally regulated and tissue-specific in leaves and flowers. Chalcone isomerase (CHI) catalyzes the formation of an intermediate common to both flavonoid pigment and isoflavonoid phytoalexin biosynthetic pathways and elevated CHI enzyme activity is correlated with the accumulation of these compounds in many plants. To determine the molecular basis for enzyme induction, we constructed a lambda gt 11 cDNA library prepared from mRNA from Phaseolus vulgaris cell cultures treated with a fungal cell wall preparation (elicitor). Two positive clones were obtained by screening 10(5) recombinants with an antiserum that had been raised against purified CHI. The identity of the cloned sequence was confirmed by showing that the largest clone (CHI 1) encoded polypeptides which were precipitated by the antiserum. Blot hybridization of RNA from elicitor treated cell cultures with labeled CHI l showed substantial accumulation of a 0.95 kb RNA within 3-4 h. The kinetics of CHI mRNA accumulation are similar to those of phenylalanine ammonia-lyase and chalcone synthase mRNAs, which encode other enzymes common to both pathways. CHI l hybridized to single fragments on genomic DNA blots suggesting a single gene. The structure and expression of the gene is being investigated to elucidate the regulatory sequences conferring developmental and environmental inducibility. (AS) |
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eng |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Phaseolus vulgaris |
| 9 (RLIN) |
eng |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Colletotrichum lindemuthianum |
| 9 (RLIN) |
eng |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
DISEASE PHYSIOLOGY AND BIOCHEMISTRY |
| 9 (RLIN) |
eng |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Tissue culture |
| 9 (RLIN) |
eng |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Genes |
| 9 (RLIN) |
eng |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
DNA |
| 9 (RLIN) |
eng |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
RNA |
| 9 (RLIN) |
eng |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Enzymes |
| 9 (RLIN) |
eng |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Phaseolus vulgaris |
| 9 (RLIN) |
spa |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Colletotrichum lindemuthianum |
| 9 (RLIN) |
spa |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
FISIOLOGIA Y BIOQUIMICA DE LAS ENFERMEDADES |
| 9 (RLIN) |
spa |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Cultivo de tejidos |
| 9 (RLIN) |
spa |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Genes |
| 9 (RLIN) |
spa |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
ADN |
| 9 (RLIN) |
spa |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
ARN |
| 9 (RLIN) |
spa |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Enzimas |
| 9 (RLIN) |
spa |
| 653 ## - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Frijol |
| -- |
spa |
| 653 ## - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Beans |
| -- |
spa |
| 653 ## - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Book chapters |
| -- |
spa |
| 653 ## - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Capítulos de libros |
| -- |
spa |
| 653 ## - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Book chapters |
| -- |
eng |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
LAMB, C.J. |
| 773 ## - HOST ITEM ENTRY |
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| Title |
In: Firtel, R.A.; Davidson, E.H. (eds.). Molecular approaches to developmental biology |
| Place, publisher, and date of publication |
New York, NY, USA, Alan R. Liss, 1987 |
| 998 ## - Data Base |
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| DataBase |
FRIJOL |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Book Chapters |