Improved enzymic assay for cyanogens in fresh and processed cassava (Record no. 77015)

MARC details
000 -LEADER
fixed length control field 05213nab a22006497a 4500
001 - CONTROL NUMBER
control field 39649
003 - CONTROL NUMBER IDENTIFIER
control field CO-PlCIA
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20130510180942.0
040 ## - CATALOGING SOURCE
Original cataloging agency CO-PlCIA
Transcribing agency CO-PlCIA
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
050 ## - LIBRARY OF CONGRESS CALL NUMBER
Classification number 41190
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 120504t1991 000 0seng d
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name O'Brien, G.M.
245 ## - TITLE STATEMENT
Title Improved enzymic assay for cyanogens in fresh and processed cassava
300 ## - PHYSICAL DESCRIPTION
Extent 56(2):277-289
520 ## - SUMMARY, ETC.
Summary, etc Se modifico el ensayo para cianogenos de yuca desarrollado por el Instituto de Recursos Naturales para asi superar algunos de los problemas encontrados cuando el ensayo se aplica a productos de la yuca. La inclusion de 25 por ciento de etanol en el medio de extraccion aumento el vol. de extracto recuperado de los productos de la yuca procesados al calor, elimino la necesidad de centrifugacion y no interfirio con ningun aspecto del ensayo. Se observo una mayor recuperacion de cianohidrina y se cambio la estimacion del contenido de cianogenos para considerar el vol. total del extracto. Se logro el examen de los 3 cianogenos (glucosido, cianohidrina y cianuro libre) por separado mediante el uso de alicuotas del extracto como tampon seguido del tratamiento apropiado. Se demostro la importancia del analisis del cianuro libre (HCN) a pH 4. Por encima de este valor de pH, la degradacion de cianohidrina tambien produce cianuro libre, suscitando valores equivocos. Se determino la eficiencia del medio de extraccion para recuperar la linamarina y la cianohidrina adicionadas a partir de productos alimenticios derivados de la yuca. Se mejoro la recuperacion de cianohidrina utilizando medio acido/etanol. Se examino la estabilidad de los cianogenos en el medio de extraccion acido/etanol a temp. ambiente y temp. de refrigeracion. Durante un periodo de 2 meses, los extractos refrigerados mostraron una variacion aceptable en comparacion con la variacion normal dentro del ensayo (5 por ciento) para los cianogenos totales y no glicosidicos pero los niveles de cianuro libre presentaron grandes disminuciones (perdidas de 15-56 por ciento). Puesto que falta determinar la toxicidad relativa de los 3 cianogenos, la cantidad relativa de cada cianogeno puede ser importante al evaluar la seguridad de los productos de la yuca. (RA-CIAT)
-- spa
520 ## - SUMMARY, ETC.
Summary, etc The assay for cassava cyanogens developed at the Natural Resources Institute has been modified to overcome some of the problems encountered when the assay is applied to cassava products. Inclusion of 25 percent ethanol in the extraction medium increased the volume of recovered extract from heat-processed cassava products, eliminated the need for centrifugation and did not interfere with any aspect of the assay. Greater cyanohydrin recovery was noted and the calculation for cyanogen contents was changed to take into account the total extract volume. The separate assay of the three cyanogens (glucosides, cyanohydrins and free cyanide) was achieved by buffering aliquots of the extract followed by appropriate treatment. The importance of assaying for free cyanide (HCN) at pH 4 was demonstrated. Above this pH, cyanohydrin degradation also produces free cyanide, giving rise to misleading values. The efficiency of the extraction medium in recovering added linamarin and cyanohydrin from cassava foods was determined. Recoveries of cyanohydrin were improved using the ethanol/acid medium. The stability of the cyanogens in the ethanol/acid extraction medium was tested at ambient and refrigeration temperatures. Over a two-month period, refrigerated extracts showed acceptable variation as compared with normal variation within the assay (5 percent) for total and non-glycosidic cyanogens but the levels of free cyanide showed heavy losses (15-56 percent lost). Since the relative toxicities of the three cyanogens have yet to be ascertained, the relative amount of each cyanogen may be important when assessing the safety of cassava products. (AS)
-- eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Manihot esculenta
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Cyanides
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Enzymes
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element HCN CONTENT
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Analysis
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element LINAMARIN
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element LINAMARASE
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element pH
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element GARI
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Storage
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Temperature
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Climatic requirements
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element COMPOSITION
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Cultivation
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element CYANOGENIC GLUCOSIDES
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element FOOD PRODUCTS
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Starch crops
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Uses
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Manihot esculenta
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Cianuros
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Enzimas
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element CONTENIDO DE HCN
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element ANALISIS
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element LINAMARINA
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element LINAMARASA
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element pH
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element GARI
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Almacenamiento
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Temperatura
9 (RLIN) spa
653 ## - INDEX TERM--UNCONTROLLED
Uncontrolled term Yuca
-- spa
653 ## - INDEX TERM--UNCONTROLLED
Uncontrolled term Artículos en revistas
-- spa
653 ## - INDEX TERM--UNCONTROLLED
Uncontrolled term Journal articles
-- spa
653 ## - INDEX TERM--UNCONTROLLED
Uncontrolled term Cassava
-- spa
653 ## - INDEX TERM--UNCONTROLLED
Uncontrolled term Journal article
-- eng
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Taylor, A.J.
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Poulter, N.H.
773 ## - HOST ITEM ENTRY
Title Journal of the Science of Food and Agriculture
Place, publisher, and date of publication 1991
998 ## - Data Base
DataBase YUCA
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Library of Congress Classification
Koha item type Journal Article
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Use restrictions Not for loan Collection code Home library Current library Shelving location Date acquired Total Checkouts Full call number Barcode Date last seen Copy number Price effective from Koha item type
    Library of Congress Classification     Short Loan Document Collection CINFOS CIAT Library CIAT Library Document collection CINFOS 05/04/2012   41190 100059652 05/04/2012 c.1 05/04/2012 Journal Article

Powered by Koha