Purification, characterization, and localization of linamarase in cassava (Record no. 77658)

MARC details
000 -LEADER
fixed length control field 04512nab a22006737a 4500
001 - CONTROL NUMBER
control field 39649
003 - CONTROL NUMBER IDENTIFIER
control field CO-PlCIA
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20130510181256.0
040 ## - CATALOGING SOURCE
Original cataloging agency CO-PlCIA
Transcribing agency CO-PlCIA
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
050 ## - LIBRARY OF CONGRESS CALL NUMBER
Classification number 37180
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 120504t1990 000 0seng d
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Mkpong, O.E.
245 ## - TITLE STATEMENT
Title Purification, characterization, and localization of linamarase in cassava
300 ## - PHYSICAL DESCRIPTION
Extent 93(1):176-181
520 ## - SUMMARY, ETC.
Summary, etc Se purifico linamarasa de yuca a homogeneidad aparente mediante un procedimiento simplificado de extraccion, utilizando tampon de fosfato de pH bajo. Se identificaron 3 isozimas de linamarasa de yuca en hojas, con base en diferencias en punto isoelectrico. La enzima puede hidrolizar varios beta-glicosidos ademas de linamarina; es inusualmente estable y posee una temp. optima de 55 grados C. Estudios de marcacion reactiva inmunologica con coloides de oro indican que la linamarasa se localiza en las paredes celulares del tejido foliar de la yuca. Ya que la linamarina debe atravesar la pared celular despues de la sintesis foliar para transportarse a la raiz, es probable que deba cruzar la pared celular en forma no hidrolizable, posiblemente como el diglucosido linustatina. Ademas, se cuantificaron los niveles de actividad de linamarina y linamarasa en hojas de var. de yuca que difieren en contenido de linamarina en sus raices. No se observaron diferencias sustanciales en el contenido de linamarina en equilibrio dinamico ni en la actividad de linamarasa de hojas de var. con alto o bajo contenido cianogenico radical. Estos resultados indican que los niveles de linamarina y linamarasa en equilibrio dinamico en hojas de var. con alto y bajo contenido cianogenico no se correlacionan con las diferencias var. en los niveles de linamarina en equilibrio dinamico en las raices. (RA-CIAT)
-- spa
520 ## - SUMMARY, ETC.
Summary, etc We have purified cassava (Manihot esculenta) linamarase to apparent homogeneity using a simplified extraction procedure using low pH phosphate buffer. Three isozymes of cassava linamarase were identified in leaves based on differences in isoelectric point. The enzyme is capable of hydrolyzing a number of beta-glycosides in addition to linamarin. The enzyme is unusually stable and has a temperature optimum of 55 degrees C. Immunogold labeling studies indicate that linamarase is localized in the cell walls of cassava leaf tissue. Since linamarin must cross the cell wall following synthesis in the leaf for transport to the root, it is likely that linamarin must cross the cell wall in a nonhydrolyzable form, possibly as the diglucoside, linustatin. In addition, we have quantified the levels of linamarin and linamarase activity in leaves of cassava varieties which differ in the linamarin content of their roots. We observed no substantial differences in the steady state linamarin content or linamarase activity of leaves from high or low (root) cyanogenic varieties. These results indicate that the steady state levels of linamarin and linamarase in leaves of high and low cyanogenic varieties are not correlated with the varietal differences in the steady state levels of linamarin in roots. (AS)
-- eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Manihot esculenta
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element CYANOGENESIS
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Leaves
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element LINAMARASE
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Hydrolysis
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element LINAMARIN
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Roots
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element HCN CONTENT
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Analysis
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element USA
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element COMPOSITION
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element CYANOGENIC GLUCOSIDES
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Enzymes
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element North America
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Plant anatomy
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Starch crops
9 (RLIN) eng
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Manihot esculenta
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element CIANOGENESIS
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Hojas
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element LINAMARASA
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Hidrolisis
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element LINAMARINA
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element RAICES
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element CONTENIDO DE HCN
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element ANALISIS
9 (RLIN) spa
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element EE.UU.
9 (RLIN) spa
653 ## - INDEX TERM--UNCONTROLLED
Uncontrolled term Journal articles
-- spa
653 ## - INDEX TERM--UNCONTROLLED
Uncontrolled term Artículos en revistas
-- spa
653 ## - INDEX TERM--UNCONTROLLED
Uncontrolled term Cassava
-- spa
653 ## - INDEX TERM--UNCONTROLLED
Uncontrolled term Yuca
-- spa
653 ## - INDEX TERM--UNCONTROLLED
Uncontrolled term CIAT MENCION
-- spa
653 ## - INDEX TERM--UNCONTROLLED
Uncontrolled term Electronic documents
-- spa
653 ## - INDEX TERM--UNCONTROLLED
Uncontrolled term Documentos electrónicos
-- spa
653 ## - INDEX TERM--UNCONTROLLED
Uncontrolled term Journal article
-- eng
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Yan, G.
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name CHISM, G.
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Sayre, R.T.
773 ## - HOST ITEM ENTRY
Title Plant Physiology
Place, publisher, and date of publication 1990
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://www.plantphysiol.org/cgi/reprint/93/1/176">http://www.plantphysiol.org/cgi/reprint/93/1/176</a>
998 ## - Data Base
DataBase YUCA
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Library of Congress Classification
Koha item type Journal Article
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Use restrictions Not for loan Collection code Home library Current library Shelving location Date acquired Total Checkouts Full call number Barcode Date last seen Copy number Price effective from Koha item type
    Library of Congress Classification     Short Loan Document Collection CINFOS CIAT Library CIAT Library Document collection CINFOS 05/04/2012   37180 100056561 05/04/2012 c.1 05/04/2012 Journal Article
    Library of Congress Classification   Restricted Access Not For Loan Electronic Document CIAT Library CIAT Library Web 05/04/2012   37180   05/04/2012   05/04/2012 Online Document

Powered by Koha