MARC details
000 -LEADER |
fixed length control field |
04512nab a22006737a 4500 |
001 - CONTROL NUMBER |
control field |
39649 |
003 - CONTROL NUMBER IDENTIFIER |
control field |
CO-PlCIA |
005 - DATE AND TIME OF LATEST TRANSACTION |
control field |
20130510181256.0 |
040 ## - CATALOGING SOURCE |
Original cataloging agency |
CO-PlCIA |
Transcribing agency |
CO-PlCIA |
041 ## - LANGUAGE CODE |
Language code of text/sound track or separate title |
eng |
050 ## - LIBRARY OF CONGRESS CALL NUMBER |
Classification number |
37180 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
120504t1990 000 0seng d |
100 ## - MAIN ENTRY--PERSONAL NAME |
Personal name |
Mkpong, O.E. |
245 ## - TITLE STATEMENT |
Title |
Purification, characterization, and localization of linamarase in cassava |
300 ## - PHYSICAL DESCRIPTION |
Extent |
93(1):176-181 |
520 ## - SUMMARY, ETC. |
Summary, etc |
Se purifico linamarasa de yuca a homogeneidad aparente mediante un procedimiento simplificado de extraccion, utilizando tampon de fosfato de pH bajo. Se identificaron 3 isozimas de linamarasa de yuca en hojas, con base en diferencias en punto isoelectrico. La enzima puede hidrolizar varios beta-glicosidos ademas de linamarina; es inusualmente estable y posee una temp. optima de 55 grados C. Estudios de marcacion reactiva inmunologica con coloides de oro indican que la linamarasa se localiza en las paredes celulares del tejido foliar de la yuca. Ya que la linamarina debe atravesar la pared celular despues de la sintesis foliar para transportarse a la raiz, es probable que deba cruzar la pared celular en forma no hidrolizable, posiblemente como el diglucosido linustatina. Ademas, se cuantificaron los niveles de actividad de linamarina y linamarasa en hojas de var. de yuca que difieren en contenido de linamarina en sus raices. No se observaron diferencias sustanciales en el contenido de linamarina en equilibrio dinamico ni en la actividad de linamarasa de hojas de var. con alto o bajo contenido cianogenico radical. Estos resultados indican que los niveles de linamarina y linamarasa en equilibrio dinamico en hojas de var. con alto y bajo contenido cianogenico no se correlacionan con las diferencias var. en los niveles de linamarina en equilibrio dinamico en las raices. (RA-CIAT) |
-- |
spa |
520 ## - SUMMARY, ETC. |
Summary, etc |
We have purified cassava (Manihot esculenta) linamarase to apparent homogeneity using a simplified extraction procedure using low pH phosphate buffer. Three isozymes of cassava linamarase were identified in leaves based on differences in isoelectric point. The enzyme is capable of hydrolyzing a number of beta-glycosides in addition to linamarin. The enzyme is unusually stable and has a temperature optimum of 55 degrees C. Immunogold labeling studies indicate that linamarase is localized in the cell walls of cassava leaf tissue. Since linamarin must cross the cell wall following synthesis in the leaf for transport to the root, it is likely that linamarin must cross the cell wall in a nonhydrolyzable form, possibly as the diglucoside, linustatin. In addition, we have quantified the levels of linamarin and linamarase activity in leaves of cassava varieties which differ in the linamarin content of their roots. We observed no substantial differences in the steady state linamarin content or linamarase activity of leaves from high or low (root) cyanogenic varieties. These results indicate that the steady state levels of linamarin and linamarase in leaves of high and low cyanogenic varieties are not correlated with the varietal differences in the steady state levels of linamarin in roots. (AS) |
-- |
eng |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Manihot esculenta |
9 (RLIN) |
eng |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
CYANOGENESIS |
9 (RLIN) |
eng |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Leaves |
9 (RLIN) |
eng |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
LINAMARASE |
9 (RLIN) |
eng |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Hydrolysis |
9 (RLIN) |
eng |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
LINAMARIN |
9 (RLIN) |
eng |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Roots |
9 (RLIN) |
eng |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
HCN CONTENT |
9 (RLIN) |
eng |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Analysis |
9 (RLIN) |
eng |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
USA |
9 (RLIN) |
eng |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
COMPOSITION |
9 (RLIN) |
eng |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
CYANOGENIC GLUCOSIDES |
9 (RLIN) |
eng |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Enzymes |
9 (RLIN) |
eng |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
North America |
9 (RLIN) |
eng |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Plant anatomy |
9 (RLIN) |
eng |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Starch crops |
9 (RLIN) |
eng |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Manihot esculenta |
9 (RLIN) |
spa |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
CIANOGENESIS |
9 (RLIN) |
spa |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Hojas |
9 (RLIN) |
spa |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
LINAMARASA |
9 (RLIN) |
spa |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Hidrolisis |
9 (RLIN) |
spa |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
LINAMARINA |
9 (RLIN) |
spa |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
RAICES |
9 (RLIN) |
spa |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
CONTENIDO DE HCN |
9 (RLIN) |
spa |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
ANALISIS |
9 (RLIN) |
spa |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
EE.UU. |
9 (RLIN) |
spa |
653 ## - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
Journal articles |
-- |
spa |
653 ## - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
ArtÃculos en revistas |
-- |
spa |
653 ## - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
Cassava |
-- |
spa |
653 ## - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
Yuca |
-- |
spa |
653 ## - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
CIAT MENCION |
-- |
spa |
653 ## - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
Electronic documents |
-- |
spa |
653 ## - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
Documentos electrónicos |
-- |
spa |
653 ## - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
Journal article |
-- |
eng |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
Yan, G. |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
CHISM, G. |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
Sayre, R.T. |
773 ## - HOST ITEM ENTRY |
Title |
Plant Physiology |
Place, publisher, and date of publication |
1990 |
856 ## - ELECTRONIC LOCATION AND ACCESS |
Uniform Resource Identifier |
<a href="http://www.plantphysiol.org/cgi/reprint/93/1/176">http://www.plantphysiol.org/cgi/reprint/93/1/176</a> |
998 ## - Data Base |
DataBase |
YUCA |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Source of classification or shelving scheme |
Library of Congress Classification |
Koha item type |
Journal Article |