MARC details
| 000 -LEADER |
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| fixed length control field |
03732nab a22005777a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
39649 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
CO-PlCIA |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20130510185040.0 |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
CO-PlCIA |
| Transcribing agency |
CO-PlCIA |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
spa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
120511t1995 000 0sspa d |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Maya, María Mercedes |
| 245 ## - TITLE STATEMENT |
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| Title |
Marcadores moleculares RAPD confirman la diversidad y evolución de Phaeoisariopsis griseola en América Latina |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
19(2):1-6 |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc |
Se evaluaron 100 aislamientos del hongo causal de la mancha angular del frijol común, Phaeoisariopsis griseola procedentes de varios países de América Latina, utilizando una variación de la técnica de la reacción en cadena de la polimerasa (PCR) consistente en la ampliación aleatoria de polimorfismos en el ADN, conocida como RAPD ("Random Amplified Polymorphic DNA"). El ADN de los aislamientos fue amplificado por PCR utilizando seis iniciadores aleatorios de diez nucleótidos, los cuales mostraron polimorfismos entre aislamientos. Se seleccionaron 44 fragmentos de ADN polimórfico amplificados para la evaluación, en la cual se utilizó un programa de parsimonia para agrupar los aislamientos genéticamente cercanos. Los resultados sugieren que el patógeno de la mancha angular ha evolucionado con grupos de acervos genéticos de frijol, corroborando y ampliando resultados previos obtenidos con variedades diferenciales, isoenzimas y polimorfismos de longitud de fragmentos de restricción (RFLPs) |
| -- |
spa |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc |
Regional pathotype diversity of the causal agent of angular leaf spot Phaeoisariopsis griseola was associated with the genetic background of common bean cultivars. To investigate the degree of genetic diversity of the pathogen, pathotype data were complemented with molecular genetic data. A variation of the polymerase chain reaction (PCR) was used to obtain random amplified polymorphic DNA (RAPD) markers. A total of 100 isolates of P. griseola from several bean growing regions in Latin America was evaluated. Total DNA from these fungal isolates was amplified utilizing six randomly selected decanucleotides that demonstrated polymorphisms between isolates. Parsimony analysis was performed on 44 polymorphic bands, producing groups of genetically related fungal isolates. The results obtained suggest that the angular leaf spot pathogen has coevolved with common bean gene pools, in agreement with previous results obtained utilizing differential varieties, isoenzyme analysis and restriction fragment length polymorphisms (RFLP) |
| -- |
eng |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Phaseolus vulgaris |
| 9 (RLIN) |
eng |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Genotypes |
| 9 (RLIN) |
eng |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Phaeoisariopsis griseola |
| 9 (RLIN) |
eng |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Genetic markers |
| 9 (RLIN) |
eng |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
DNA |
| 9 (RLIN) |
eng |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Polymorphism |
| 9 (RLIN) |
eng |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Latin America |
| 9 (RLIN) |
eng |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Phaseolus vulgaris |
| 9 (RLIN) |
spa |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Genotipos |
| 9 (RLIN) |
spa |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Phaeoisariopsis griseola |
| 9 (RLIN) |
spa |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Marcadores genéticos |
| 9 (RLIN) |
spa |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
ADN |
| 9 (RLIN) |
spa |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Polimorfismo |
| 9 (RLIN) |
spa |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
América Latina |
| 9 (RLIN) |
spa |
| 653 ## - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Thompson |
| -- |
spa |
| 653 ## - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
RJ |
| -- |
spa |
| 653 ## - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Beans |
| -- |
spa |
| 653 ## - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Frijol |
| -- |
spa |
| 653 ## - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
RAPD |
| -- |
spa |
| 653 ## - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
CIAT Autor |
| -- |
spa |
| 653 ## - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Genética vegetal y fitomejoramiento |
| -- |
spa |
| 653 ## - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Enfermedades de las plantas |
| -- |
spa |
| 653 ## - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
RAPD |
| -- |
eng |
| 653 ## - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Plant genetics and breeding |
| -- |
eng |
| 653 ## - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Plant diseases |
| -- |
eng |
| 653 ## - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Articles in Refereed Journals |
| -- |
eng |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Otoya, María Mercedes |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Mayor, Jorge Edgard |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Pastor Corrales, Marcial Antonio |
| 773 ## - HOST ITEM ENTRY |
|---|
| Title |
Fitopatología Colombiana (Colombia) |
| Place, publisher, and date of publication |
1995 |
| 998 ## - Data Base |
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| DataBase |
CATAL |
| 998 ## - Data Base |
|---|
| DataBase |
FRIJOL |
| 998 ## - Data Base |
|---|
| DataBase |
BICIAT |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Journal Article |