DNA microsatellites for the development of reliable PCR-based length polymorphisms in Phaseolus
Material type: ArticleLanguage: English Description: p. 76-80Subject(s):- Phaseolus vulgaris
- Genetic polymorphism
- Genetic markers
- Nucleases
- Colombia
- Phaseolus vulgaris
- Polimorfismo genético
- Marcadores genéticos
- Nucleasas
- Colombia
- Beans
- CIAT Editor
- Frijol
- Articles in proceedings
- Artículos en memorias
- Genética vegetal y fitomejoramiento
- Plant genetics and breeding
- Articles in Proceedings
- SB 327 .P5
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Book Chapters | CIAT Library CIAT's Historical Collection | CIAT's Historical Collection | SB 327 .P5 (Browse shelf(Opens below)) | c.1 | Not For Loan (Restricted Access) |
DNA microsatellites or Simple Sequence Repeats (SSR), consist of very short DNA sequences of repeated nucleotide motifs. Microsatellites were found to be abundant in human, mouse and more recently in rice, tomato and soybean. They have been shown to detect a high level of polymorphism making them ideal in the fine mapping of tagged genes, the detection of polymorphism in intraspecific crosses and population genetic studies. We have initiated a project to assess the presence, abundance of microsatellites in common bean and their potential as markers in mapping. A bean DNA genomic library was constructed by digesting total DNA to completion with Sau 3AI and cloning the fragments of less than 500 bp into the Xho I site of Lambda ZAP II. The library was screened with synthetic oligonucleotides, composed of di, tri or tetranucleotides repeats 8-18 times. The oligonucleotides were selected from previous reports on plant microsatellites or designed for this study. A first screening of the library using these oligonucleotides suggests that similar to other plant species CA repeats are present at very low frequency in bean in constrast with the human genome. (ATT)n and (AT)n repeats are so far the most abundant. Work is in progress to purify positive clones, sequence them, and design specific PCR primers flanking the microsatellites for use as new molecular markers in bean mapping. eng