Whitefly transmission and efficient ssDNA accumulation of bean golden mosaic geminivirus require functional coat protein
Material type:
- Phaseolus vulgaris
- Bemisia tabaci
- Plant viruses
- Genes
- DNA
- Geminiviruses
- Disease transmission
- Proteins
- Phaseolus vulgaris
- Bemisia tabaci
- Virus de las plantas
- Genes
- ADN
- Geminivirus
- Transmisión de enfermedades
- Proteínas
- Beans
- Frijol
- Journal articles
- Artículos en revistas
- Virus del mosaico dorado del frijol
- Enfermedades de las plantas
- Plagas de las Plantas
- Bean golden mosaic virus
- Plant diseases
- Pests of plants
- Journal article
Item type | Current library | Collection | Call number | Copy number | Status | Date due | Barcode | Item holds | |
---|---|---|---|---|---|---|---|---|---|
![]() |
CIAT Library Journal Collection | Journal Collection | c.1 | Not For Loan (Restricted Access) |
Bean golden mosaic geminivirus (BGMV) has a bipartite genome composed of two circular ssDNA components (DNA-A and DNA-B) and is transmitted by the whitefly, Bemisia tabaci. DNA-A encodes the viral replication proteins and the coat protein. To determine the role of BGMV coat protein in systemic infection and whitefly transmission, two deletions and a restriction fragment inversion were introduced into the BGMV coat protein gene. All three coat protein mutants produced systemic infections when coinoculated with DNA-B onto Phaseolus vulgaris using electric discharge particle acceleration "particle gun." However, they were not sap transmissible and coat protein was not detected in mutant-infected plants. In addition, none of the mutants were transmitted by whiteflies. With all three mutants, ssDNA accumulation of DNA-A and DNA-B was reduced 25- to 50-fold and 3- to 10-fold, respectively, as compared to that of wild-type DNA. No effect on dsDNA-A accumulation was detected and there was 2- to 5-fold increase in dsDNA-B accumulation. Recombinants between the mutated DNA-A and DNA-B forms were identified when the inoculated coat protein mutant was linearized in the common region eng