Image from Google Jackets

Detection of Indian cassava mosaic virus by ELISA

By: Contributor(s): Material type: ArticleArticleLanguage: English Description: 58(3):149-152Subject(s): LOC classification:
  • 38417
Online resources: In: Current ScienceSummary: Se utilizo la tecnica ELISA de doble sandwich para detectar el virus del mosaico hindu de la yuca y para determinar su confiabilidad como un metodo efectivo de seleccion de material de siembra sano. Para el ensayo, se utilizaron hojas jovenes completamente expandidas de plantas naturalmente infectadas de los cv. de yuca Kalikalan, H-226 y H-1687; todas las plantas presentaron sintomas severos. Tambien se usaron hojas de plantas aparentemente sanas en el campo, que habian sido derivadas de cultivos de meristemos. El virus se detecto facilmente en los 3 cv. en las hojas de plantas infectadas que presentaron sintomas tipicos. Tambien se detecto el virus del mosaico hindu de la yuca en algunas plantas aparentemente sanas, lo cual indica una infeccion reciente de las mismas. Existe un gran riesgo de que los agricultores, al recolectar material de siembra de estas plantas aparentemente sanas, proporcionen una fuente de inoculo en siembras nuevas. Estos resultados, por tanto, indican que la tecnica ELISA se puede utilizar efectivamente en programas de certificacion de material de siembra sano. (CIAT)Summary: The double anti-body sandwich ELISA was used to detect the cassava Indian mosaic virus and its reliability as an effective screening method to diagnose healthy planting material was determined. Young and fully expanded leaves from naturally infected plants of cassava cv. Kalikalan, H-226, and H-1687, showing severe symptoms, were tested; leaves of meristem-derived apparently healthy plants in the field were also assayed. The virus was readily detected in leaves from diseased plants of all 3 cv. showing typical symptoms. The cassava Indian mosaic virus was also detected in some of the apparently healthy plants, indicating fresh infection of the plants. There exists a risk that when farmers collect planting material from these apparently healthy plants they may provide a source of inoculum in the new plantings. These results therefore indicate that ELISA tests can be effectively used for certification programs for healthy planting material. (CIAT)
Tags from this library: No tags from this library for this title.
Star ratings
    Average rating: 0.0 (0 votes)

Se utilizo la tecnica ELISA de doble sandwich para detectar el virus del mosaico hindu de la yuca y para determinar su confiabilidad como un metodo efectivo de seleccion de material de siembra sano. Para el ensayo, se utilizaron hojas jovenes completamente expandidas de plantas naturalmente infectadas de los cv. de yuca Kalikalan, H-226 y H-1687; todas las plantas presentaron sintomas severos. Tambien se usaron hojas de plantas aparentemente sanas en el campo, que habian sido derivadas de cultivos de meristemos. El virus se detecto facilmente en los 3 cv. en las hojas de plantas infectadas que presentaron sintomas tipicos. Tambien se detecto el virus del mosaico hindu de la yuca en algunas plantas aparentemente sanas, lo cual indica una infeccion reciente de las mismas. Existe un gran riesgo de que los agricultores, al recolectar material de siembra de estas plantas aparentemente sanas, proporcionen una fuente de inoculo en siembras nuevas. Estos resultados, por tanto, indican que la tecnica ELISA se puede utilizar efectivamente en programas de certificacion de material de siembra sano. (CIAT) spa

The double anti-body sandwich ELISA was used to detect the cassava Indian mosaic virus and its reliability as an effective screening method to diagnose healthy planting material was determined. Young and fully expanded leaves from naturally infected plants of cassava cv. Kalikalan, H-226, and H-1687, showing severe symptoms, were tested; leaves of meristem-derived apparently healthy plants in the field were also assayed. The virus was readily detected in leaves from diseased plants of all 3 cv. showing typical symptoms. The cassava Indian mosaic virus was also detected in some of the apparently healthy plants, indicating fresh infection of the plants. There exists a risk that when farmers collect planting material from these apparently healthy plants they may provide a source of inoculum in the new plantings. These results therefore indicate that ELISA tests can be effectively used for certification programs for healthy planting material. (CIAT) eng

Powered by Koha