Idendfication of a severe bean strain of tobacco ringspot virus isolated from white beans Phaseolus vulgaris L

By:

Tu, J.C

Material type: Article

ArticleLanguage: English Description: 101(2):153-162Subject(s): LOC classification:

16787

In: Phytopattiologische ZeitschriftSummary: Se ha identificado un aislamiento de un virus de frijol blanco infectado severamente en el sur de Ontario, Canada, como una cepa severa (SB) del virus de la mancha anular del tabaco (TRSV, criptograma R/1: 2.2/40: S/S: S/Nc). La identificacion se realizo con base en la sintomatologia, plantas indicadoras, propiedades fisicas, serologia, modo de transmision y microscopia electronica. Durante el estado inicial de infeccion la TRSV-SB causo quemazon de las yemas en plantas inoculadas de frijol blanco. Las plantas que se recuperaron de este trastorno inicial produjeron hojas deformes con un mosaico. El virus produjo una mancha anular tipica en varias especies de tabaco y reacciono positivamente al antisuero de TRSV. Las particulas de virus se identificaron en el microscopio electronico mediante tincion negativa. Las propiedades fisicas del virus en savia cruda fueron semejantes a las reportadas por otros. El virus no se transmite por semilla en el frijol blanco. Las respuestas de plantas indicadoras a la TRSV-SB indican que este virus es diferente al TRSV-B registrado previamente, el cual tiene un leve efecto en el frijol blanco, y el TRSV que afecta severamente la soya. (RA-CIAT)Summary: An isolate of a virus from severely diseased white beans in S. Ontario, Canada has been identified as a severe bean strain (SB) of tobacco ringspot virus (TRSV, cryptogram R/1: 2.2/40: S/S: S/Nc). The identification was based on symptomatology, indicator plants, physical properties, serology, mode of transmission, and electron microscopy. During the initial stage of infection, TRSV-SB caused bud blight in inoculated white beans. Plants which recovered from this initial shock produced deformed leaves with a mosaic. The virus produced typical ringspot in several species of tobacco and reacted positively to TRSV antiserum. The virus particles were discernible in the electron microscope by negative staining. Physical properties of the virus in crude sap were similar to those reported by others. The virus is not seed transmissible in white beans. The responses of the indicator plants to TRSV-SB suggest that this virus is different from the previously reported TRSV-B, which has a mild effect on white beans, and TRSV which severely affects soybeans. (AS)

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Holdings
Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode	Item holds
Journal Article	CIAT Library Document collection CINFOS	Document Collection CINFOS	16787 (Browse shelf(Opens below))	c.1	Short Loan		100064330

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Se ha identificado un aislamiento de un virus de frijol blanco infectado severamente en el sur de Ontario, Canada, como una cepa severa (SB) del virus de la mancha anular del tabaco (TRSV, criptograma R/1: 2.2/40: S/S: S/Nc). La identificacion se realizo con base en la sintomatologia, plantas indicadoras, propiedades fisicas, serologia, modo de transmision y microscopia electronica. Durante el estado inicial de infeccion la TRSV-SB causo quemazon de las yemas en plantas inoculadas de frijol blanco. Las plantas que se recuperaron de este trastorno inicial produjeron hojas deformes con un mosaico. El virus produjo una mancha anular tipica en varias especies de tabaco y reacciono positivamente al antisuero de TRSV. Las particulas de virus se identificaron en el microscopio electronico mediante tincion negativa. Las propiedades fisicas del virus en savia cruda fueron semejantes a las reportadas por otros. El virus no se transmite por semilla en el frijol blanco. Las respuestas de plantas indicadoras a la TRSV-SB indican que este virus es diferente al TRSV-B registrado previamente, el cual tiene un leve efecto en el frijol blanco, y el TRSV que afecta severamente la soya. (RA-CIAT) spa

An isolate of a virus from severely diseased white beans in S. Ontario, Canada has been identified as a severe bean strain (SB) of tobacco ringspot virus (TRSV, cryptogram R/1: 2.2/40: S/S: S/Nc). The identification was based on symptomatology, indicator plants, physical properties, serology, mode of transmission, and electron microscopy. During the initial stage of infection, TRSV-SB caused bud blight in inoculated white beans. Plants which recovered from this initial shock produced deformed leaves with a mosaic. The virus produced typical ringspot in several species of tobacco and reacted positively to TRSV antiserum. The virus particles were discernible in the electron microscope by negative staining. Physical properties of the virus in crude sap were similar to those reported by others. The virus is not seed transmissible in white beans. The responses of the indicator plants to TRSV-SB suggest that this virus is different from the previously reported TRSV-B, which has a mild effect on white beans, and TRSV which severely affects soybeans. (AS) eng