Immunochemical detection of ds-RNA in healthy and virus- infected plants and specific detection of viral ds-RNA by hybridization to labelled complementary DNA

Se detecto ARN de cadena doble (cd) en preparaciones de acido nucleico provenientes de tejidos foliares libres de virus de varias especies de plantas, mediante ensayos radiales de difusion doble con antisuero erigido en contraste al acido poliinosinico: policitidilico (poli(I):poli(C)). No se detectaron diferencias significativas en la concn. de ARN de cd en tales ensayos en hojas de habichuela cv. Hawkesbury Wonder libres de virus y en aquellas infectadas con virus del mosaico del tabaco o con virus de la mancha anular del tabaco. Sin embargo, se detectaron facilmente ARN de cd con especificidad al virus y ARN genomico de ambos virus, los que se estimaron cuantitativamente mediante hibridacion en moldes de ADN complementario activado con H3. Se concluye que no es factible evaluar materiales vegetales infectados con virus ARN de una sola cadena mediante ensayos inmunoquimicos para ARN de cd asociados al virus. (RA-CIAT) spa

Double-stranded (ds) RNA was detected in nucleic acid preparations from virus-free leaf tissues of several plant species by radial double-diffusion tests with antiserum raised against polyinosinic:polycytidylic acid [poly(I):poly(C)]. No significant differences in the concn. of ds-RNA were detected by such tests in virus-free French bean cv. Hawkesbury Wonder leaves and those infected with tobacco mosaic virus or tobacco ringspot virus. However, virus-specific ds-RNAs as well as genomic RNAs of both viruses were readily detected and estimated quantitatively by hybridization to 3H-labelled complementary DNA probes. It is concluded that it is not feasible to screen plant material infected with single-stranded RNA viruses by immunochemical tests for virus- associated ds-RNAs. (AS) eng