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Transformation of phenoxyacetic acid herbicides by Phaseolus vulgaris L. callus cells

By: Contributor(s): Material type: ArticleArticleLanguage: English Description: 3(1):5-7Subject(s): LOC classification:
  • 27492
 In: Plant Cell ReportsSummary: El herbicida auxina MCPA fue absorbido por celulas de callo de Phaseolus vulgaris en cultivo liquido y luego se hidroxilo en el grupo metilo. El alcohol permanecio en gran parte sin conjugar y se libero parcialmente hacia el medio de cultivo. Como metabolitos menores aparecieron varios glicosidos y un conjugado de MCPA soluble en eter. El acido fenoxiacetico no substituido fue metabolizado primordialmente por hidroxilacion-4 y la formacion posterior de glucosido fenolico. Las celulas retuvieron los metabolitos del acido fenoxiacetico. En el caso de ambos substratos, el metabolismo en las celulas se correlaciono bien con el observado con yemas apicales cortadas de plantulas ahiladas. (RA- CIAT)Summary: The auxin herbicide MCPA was absorbed by liquid-cultured callus cells of Phaseolus vulgaris and subsequently became hydroxylated at the methyl group. The alcohol remained largely unconjugated and was partly released to the culture medium. Several glycosides and an ether-soluble conjugate of MCPA appeared as minor metabolites. Unsubstituted phenoxyacetic acid was metabolized primarily by 4- hydroxylation and subsequent phenolic glucoside formation. Metabolites of phenoxyacetic acid were retained by the cells. In the case of both substrates, metabolism in cells correlated well with that observed in apical buds excised from etiolated seedlings. (AS)
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Journal Article Journal Article CIAT Library Document collection CINFOS Document Collection CINFOS 27492 (Browse shelf(Opens below)) c.1 Short Loan 100062486
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El herbicida auxina MCPA fue absorbido por celulas de callo de Phaseolus vulgaris en cultivo liquido y luego se hidroxilo en el grupo metilo. El alcohol permanecio en gran parte sin conjugar y se libero parcialmente hacia el medio de cultivo. Como metabolitos menores aparecieron varios glicosidos y un conjugado de MCPA soluble en eter. El acido fenoxiacetico no substituido fue metabolizado primordialmente por hidroxilacion-4 y la formacion posterior de glucosido fenolico. Las celulas retuvieron los metabolitos del acido fenoxiacetico. En el caso de ambos substratos, el metabolismo en las celulas se correlaciono bien con el observado con yemas apicales cortadas de plantulas ahiladas. (RA- CIAT) spa

The auxin herbicide MCPA was absorbed by liquid-cultured callus cells of Phaseolus vulgaris and subsequently became hydroxylated at the methyl group. The alcohol remained largely unconjugated and was partly released to the culture medium. Several glycosides and an ether-soluble conjugate of MCPA appeared as minor metabolites. Unsubstituted phenoxyacetic acid was metabolized primarily by 4- hydroxylation and subsequent phenolic glucoside formation. Metabolites of phenoxyacetic acid were retained by the cells. In the case of both substrates, metabolism in cells correlated well with that observed in apical buds excised from etiolated seedlings. (AS) eng

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