Subunit studies of coupling factor 1 of bean chloroplasts

By:

SILVANOVICH, M.P

Contributor(s):

HILL, R.D

Material type: Article

ArticleLanguage: English Description: 54:481-487Subject(s):

LOC classification:

7973

In: Canadian Journal of BiochemistrySummary: Se estudio un factor acoplador (CF1) del cloroplasto del frijol, con un Ca(2+) dependiente de la actividad de la ATPasa. La inmunodifusion del cloroplasto del frijol (Phaseolus vulgaris) y los factores acopladores del etioplasto, y el factor acoplador de la espinaca contra el antisuero para el factor acoplador de la espinaca, mostraron una identidad parcial del factor acoplador del frijol con el de la espinaca. Una comparacion inmunoelectroforetica, bajo condiciones de disociacion de extractos de hojas de frijol y de extractos de espinaca que contenia subunidades de CF1 (en base a la determinacion mediante electroforesis de gel de poliacrilamida sulfato dodecil de sodio), dio resultados identicos para ambos extractos. Se encontraron por lo menos 6 especies distintas de polipeptidos. Las especies mas grandes tuvieron un peso molecular de 42.000, 59.000 y 63.000 daltons. El analisis de aminoacidos de CF1 de frijol purificado electroforeticamente, dio resultados similares a los ya publicados sobre el CF1 de la espinaca. (RA-CIAT)Summary: A bean chloroplast coupling factor (CF1) with latent Ca(2)+- dependent ATPase acitvity was studied. Immunodiffusion of bean (Phaseolus vulgaris) chloroplast and etioplast coupling factors and spinach coupling factor against atiserum to spinach coupling factor showed partial identity of the bean coupling factor with that of spinach. An immunoelectrophoretic comparison, under dissociating conditions, of bean leaf extracts and spinach extracts containing CF1 subunits (as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis) gave identical results for both extracts. At least 6 distinct polypeptide species were found. The major species had a mol wt of 42000, 59000 and 63000 daltons. Amino acid analysis of electrophoretically purified bean CF1 gave results similar to those published for spinach CF1. (AS)

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Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode	Item holds
Journal Article	CIAT Library Document collection CINFOS	Document Collection CINFOS	7973 (Browse shelf(Opens below))	c.1	Short Loan		100066557

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Previous	7970 Determinacao da epoca adequada de colheita do feijao (Phaseolus vulgaris L.) com base na qualidade fisiologica das sementes	7971 Qualidade fisiologica das sementes de feijao (Phaseolus vulgaris L.) colhidas em diferentes periodos apos a fecundacao do ovulo	7972 In vitro simulation of cytoplasmic membrane senescence in cotyledons	7973 Subunit studies of coupling factor 1 of bean chloroplasts	7974 Estudio del comportamiento productivo de variedades de porotos (Phaseolus vulgaris) para exportacion. Tesis (Ingeniero Agrónomo)	7975 Caracterizacion de la respuesta a la fertilizacion (N-P-K) en poroto (Phaseolus vulgaris L.). Tesis (Ingeniero Agrónomo)	7976 A study in East Africa of the bean flies (Dip., Agromyzidae) affecting Phaseolus vulgaris and of their natural enemies, with the description of a new species of Melanogromyza Hend	Next

Se estudio un factor acoplador (CF1) del cloroplasto del frijol, con un Ca(2+) dependiente de la actividad de la ATPasa. La inmunodifusion del cloroplasto del frijol (Phaseolus vulgaris) y los factores acopladores del etioplasto, y el factor acoplador de la espinaca contra el antisuero para el factor acoplador de la espinaca, mostraron una identidad parcial del factor acoplador del frijol con el de la espinaca. Una comparacion inmunoelectroforetica, bajo condiciones de disociacion de extractos de hojas de frijol y de extractos de espinaca que contenia subunidades de CF1 (en base a la determinacion mediante electroforesis de gel de poliacrilamida sulfato dodecil de sodio), dio resultados identicos para ambos extractos. Se encontraron por lo menos 6 especies distintas de polipeptidos. Las especies mas grandes tuvieron un peso molecular de 42.000, 59.000 y 63.000 daltons. El analisis de aminoacidos de CF1 de frijol purificado electroforeticamente, dio resultados similares a los ya publicados sobre el CF1 de la espinaca. (RA-CIAT) spa

A bean chloroplast coupling factor (CF1) with latent Ca(2)+- dependent ATPase acitvity was studied. Immunodiffusion of bean (Phaseolus vulgaris) chloroplast and etioplast coupling factors and spinach coupling factor against atiserum to spinach coupling factor showed partial identity of the bean coupling factor with that of spinach. An immunoelectrophoretic comparison, under dissociating conditions, of bean leaf extracts and spinach extracts containing CF1 subunits (as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis) gave identical results for both extracts. At least 6 distinct polypeptide species were found. The major species had a mol wt of 42000, 59000 and 63000 daltons. Amino acid analysis of electrophoretically purified bean CF1 gave results similar to those published for spinach CF1. (AS) eng