Location of curly top virus antigen in bean, sugarbeet, tobacco, and tomato by fluorescent antibody staining

By:

MUMFORD, D.L

Contributor(s):

THORNLEY, W.R

Material type: Article

ArticleLanguage: English Description: 67(10):1313-1315Subject(s): LOC classification:

9179

Online resources:

Click here to access online

In: PhytopathologySummary: Con el objeto de localizar el antigeno del virus del apice rizado en frijol, remolacha, tabaco y tomate se empleo el metodo de solucion colorante fluorecente para determinar anticuerpos. en el tejido del floema. la aparicion de sintomas foliares. 8 veces mas sitios especificamente coloreados que las plantas resistentes. se observo coloracion localizada en saltahojas viruliferos aunque si se detecto una fluorescencia generalizada.Summary: The fluorescent antibody staining method was used to locate curly top virus antigen in beans, sugarbeets, tobacco and tomatoes. Antigen was found only in phloem tissue. The occurrence of specific staining corresponded to the appearance of foliar symptoms. Susceptible tomato plants had more than 8 times as many specifically stained sites as did resistant plants. No localized staining was observed in viruliferous leafhoppers although a generalized fluorescence was detected. (AS)

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Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode	Item holds
Online Document	CIAT Library Web	Electronic Document	9179 (Browse shelf(Opens below))		Not For Loan (Restricted Access)
Journal Article	CIAT Library Document collection CINFOS	Document Collection CINFOS	9179 (Browse shelf(Opens below))	c.1	Short Loan		100050456

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Previous	9150 Metodologia parcial de analisis proyecto "desarrollo del gusano cachon en la yuca"	9177 Control of Pythium blight on bean with ethazol and prothiocarb	9178 Southern bean mosaic virus: Evidence for seed transmission in bean embryos	9179 Location of curly top virus antigen in bean, sugarbeet, tobacco, and tomato by fluorescent antibody staining	9189 Curly dwarf mosaic disease of beans from El Salvador	9190 An unusual viruslike particle associated with golden yellow mosaic of beans	9231 Involvement of phaseolotoxin in halo blight of beans: Transport and conversion to functional toxin	Next

Con el objeto de localizar el antigeno del virus del apice rizado en frijol, remolacha, tabaco y tomate se empleo el metodo de solucion colorante fluorecente para determinar anticuerpos. en el tejido del floema. la aparicion de sintomas foliares. 8 veces mas sitios especificamente coloreados que las plantas resistentes. se observo coloracion localizada en saltahojas viruliferos aunque si se detecto una fluorescencia generalizada. spa

The fluorescent antibody staining method was used to locate curly top virus antigen in beans, sugarbeets, tobacco and tomatoes. Antigen was found only in phloem tissue. The occurrence of specific staining corresponded to the appearance of foliar symptoms. Susceptible tomato plants had more than 8 times as many specifically stained sites as did resistant plants. No localized staining was observed in viruliferous leafhoppers although a generalized fluorescence was detected. (AS) eng