An improved Mathur's medium for growth, sporulation, and germination of spores of Colletotrichum lindemuthianum

By:

Tu, J.C

Material type: Article

ArticleLanguage: English Description: 44(178):87-93Subject(s): LOC classification:

29148

In: MicrobiosSummary: Se cultivo la raza delta de Colletotrichum lindemuthianum en medio de Mathur a diferentes pH, fotoperiodos y concn. de dextrosa, extracto de levadura y agar en condiciones de lab., en un intento de mejorar la esporulacion y estabilidad del cultivo. Se estudiaron el crecimiento, la esporulacion y la germinacion de las esporas funginas con respecto a factores nutricionales y de cultivo. Se formulo un medio de Mathur mejorado, el cual satisface los requerimientos para el crecimiento, la esporulacion y la germinacion de las esporas. Contiene dextrosa (11.2 g), MgSO4.7H2O (2.5 g), KH2PO4 (2.7 g), Bacto peptona (1.0 g), extracto de levadura (0.8 g), Bacto agar (12 g) y 1 litro de agua. El medio presento al final un pH de 5.5. La incubacion de las cajas fue mejor en oscuridad total a temp. ambiente (22 grados centigrados). Para resultados optimos se necesito una cantidad de 25 ml de agar/caja de Petri de tamano estandar (10 cm). Estos factores nutricionales y de cultivo han sido usados exitosamente durante los 2 anos anteriores para conservar cultivos de C. lindemuthianum con una alta produccion y viabilidad de esporas. (RA-CIAT)Summary: The race delta of Colletotrichum lindemuthianum was grown on Mathur's medium at different pH, day length, and concn. of dextrose, yeast extract, and agar under lab. conditions, in an attempt to improve sporulation and culture stability. Growth, sporulation, and germination of spores of the fungus were monitored relative to nutritional and cultural factors. An improved Mathur's medium, which met the requirements for growth, sporulation, and germination of spores, was formulated. It contained dextrose, 11.2 g; MgSO4.7H2O, 2.5 g; KH2PO4, 2.7 g; Bacto peptone, 1.0 g; yeast extract, 0.8 g; Bacto agar, 12 g; and 1 liter of water. The medium had a final pH of 5.5. Incubation of plates was best in total darkness at room temp. (22 degrees Celsius). For optimum results, 25 ml of agar/standard size (10 cm) Petri plate was needed. These nutritional and cultural factors have been successfully used for the past 2 yr to maintain cultures of C. lindemuthianum with high spore production and viability. (AS)

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Holdings
Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode	Item holds
Journal Article	CIAT Library Document collection CINFOS	Document Collection CINFOS	29148 (Browse shelf(Opens below))	c.1	Short Loan		100048582

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Se cultivo la raza delta de Colletotrichum lindemuthianum en medio de Mathur a diferentes pH, fotoperiodos y concn. de dextrosa, extracto de levadura y agar en condiciones de lab., en un intento de mejorar la esporulacion y estabilidad del cultivo. Se estudiaron el crecimiento, la esporulacion y la germinacion de las esporas funginas con respecto a factores nutricionales y de cultivo. Se formulo un medio de Mathur mejorado, el cual satisface los requerimientos para el crecimiento, la esporulacion y la germinacion de las esporas. Contiene dextrosa (11.2 g), MgSO4.7H2O (2.5 g), KH2PO4 (2.7 g), Bacto peptona (1.0 g), extracto de levadura (0.8 g), Bacto agar (12 g) y 1 litro de agua. El medio presento al final un pH de 5.5. La incubacion de las cajas fue mejor en oscuridad total a temp. ambiente (22 grados centigrados). Para resultados optimos se necesito una cantidad de 25 ml de agar/caja de Petri de tamano estandar (10 cm). Estos factores nutricionales y de cultivo han sido usados exitosamente durante los 2 anos anteriores para conservar cultivos de C. lindemuthianum con una alta produccion y viabilidad de esporas. (RA-CIAT) spa

The race delta of Colletotrichum lindemuthianum was grown on Mathur's medium at different pH, day length, and concn. of dextrose, yeast extract, and agar under lab. conditions, in an attempt to improve sporulation and culture stability. Growth, sporulation, and germination of spores of the fungus were monitored relative to nutritional and cultural factors. An improved Mathur's medium, which met the requirements for growth, sporulation, and germination of spores, was formulated. It contained dextrose, 11.2 g; MgSO4.7H2O, 2.5 g; KH2PO4, 2.7 g; Bacto peptone, 1.0 g; yeast extract, 0.8 g; Bacto agar, 12 g; and 1 liter of water. The medium had a final pH of 5.5. Incubation of plates was best in total darkness at room temp. (22 degrees Celsius). For optimum results, 25 ml of agar/standard size (10 cm) Petri plate was needed. These nutritional and cultural factors have been successfully used for the past 2 yr to maintain cultures of C. lindemuthianum with high spore production and viability. (AS) eng