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Host range and serological properties of two potyvirus isolates from Phaseolus vulgaris in Lebanon

By: Contributor(s): Material type: ArticleArticleLanguage: English Description: 19:187-194Subject(s): LOC classification:
  • 28901
In: Tropical Agriculture Research SeriesSummary: Dos aislamientos de virus obtenidos de Phaseolus vulgaris en el Libano se identificaron como potyvirus con estrecha relacion serologica entre si, con el virus del mosaico del caupi `ojo negro', con BCMV-NY 15 y con el virus del mosaico del frijol adzuki (AzMV). Ambos aislamientos infectaron 6 especies de plantas pertenecientes a 3 familias. Los 2 aislamientos se diferenciaron por su reaccion en 6 lineas IITA-TVu de caupi como tambien en 7 cv. de P. vulgaris. En pruebas ELISA de doble sandwich ambos antigenos virales reaccionaron fuertemente con antisueros del virus del mosaico del caupi `ojo negro', BCMV-NY 15 y AzMV, y debilmente con antisueros del virus del mosaico del caupi transmitido por afidos, BYMV y algunos aislamientos del BCMV. En pruebas de ELISA reciprocas, los antisueros para estos 2 virus reaccionaron fuertemente con el virus del mosaico del caupi `ojo negro' y BCMV- NY 15. Las pruebas de microscopia electronica inmunosorbente confirmaron los resultados obtenidos con ELISA; se presentaron reacciones fuertes cuando ambos aislamientos se probaron con antisueros del virus del mosaico del caupi `ojo negro', BCMV-NY 15 y AzMV. (RA-CIAT)Summary: Two virus isolates obtained from Phaseolus vulgaris in Lebanon were identified as potyviruses with close serological relationship to each other, to blackeye cowpea mosaic virus, to the BCMV-NY 15, and to adzuki bean mosaic virus (AzMV). Both isolates infected 6 plant species belonging to 3 families. The 2 isolates were differentiated from each other by their reaction on 6 IITA-TVu cowpea lines as well as on 7 P. vulgaris cv. In double antibody sandwich ELISA both virus antigens strongly reacted with blackeye cowpea mosaic virus, BCMV-NY 15, and AzMV antisera and weakly with antisera to cowpea aphid-borne mosaic virus, BYMV, and some isolates of BCMV. In reciprocal ELISA tests, antisera to these 2 virus isolates reacted strongly with blackeye cowpea mosaic virus and BCMV-NY 15. Immunosorbent electron microscopy tests confirmed the ELISA results, giving strong reactions when both isolates were tested with antisera to blackeye cowpea mosaic virus, BCMV-NY 15, and AzMV. (AS)
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Journal Article Journal Article CIAT Library Document collection CINFOS Document Collection CINFOS 28901 (Browse shelf(Opens below)) c.1 Short Loan 100064259
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Dos aislamientos de virus obtenidos de Phaseolus vulgaris en el Libano se identificaron como potyvirus con estrecha relacion serologica entre si, con el virus del mosaico del caupi `ojo negro', con BCMV-NY 15 y con el virus del mosaico del frijol adzuki (AzMV). Ambos aislamientos infectaron 6 especies de plantas pertenecientes a 3 familias. Los 2 aislamientos se diferenciaron por su reaccion en 6 lineas IITA-TVu de caupi como tambien en 7 cv. de P. vulgaris. En pruebas ELISA de doble sandwich ambos antigenos virales reaccionaron fuertemente con antisueros del virus del mosaico del caupi `ojo negro', BCMV-NY 15 y AzMV, y debilmente con antisueros del virus del mosaico del caupi transmitido por afidos, BYMV y algunos aislamientos del BCMV. En pruebas de ELISA reciprocas, los antisueros para estos 2 virus reaccionaron fuertemente con el virus del mosaico del caupi `ojo negro' y BCMV- NY 15. Las pruebas de microscopia electronica inmunosorbente confirmaron los resultados obtenidos con ELISA; se presentaron reacciones fuertes cuando ambos aislamientos se probaron con antisueros del virus del mosaico del caupi `ojo negro', BCMV-NY 15 y AzMV. (RA-CIAT) spa

Two virus isolates obtained from Phaseolus vulgaris in Lebanon were identified as potyviruses with close serological relationship to each other, to blackeye cowpea mosaic virus, to the BCMV-NY 15, and to adzuki bean mosaic virus (AzMV). Both isolates infected 6 plant species belonging to 3 families. The 2 isolates were differentiated from each other by their reaction on 6 IITA-TVu cowpea lines as well as on 7 P. vulgaris cv. In double antibody sandwich ELISA both virus antigens strongly reacted with blackeye cowpea mosaic virus, BCMV-NY 15, and AzMV antisera and weakly with antisera to cowpea aphid-borne mosaic virus, BYMV, and some isolates of BCMV. In reciprocal ELISA tests, antisera to these 2 virus isolates reacted strongly with blackeye cowpea mosaic virus and BCMV-NY 15. Immunosorbent electron microscopy tests confirmed the ELISA results, giving strong reactions when both isolates were tested with antisera to blackeye cowpea mosaic virus, BCMV-NY 15, and AzMV. (AS) eng

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