Host range and serological properties of two potyvirus isolates from Phaseolus vulgaris in Lebanon

By:

MAKKOUK, K.M

Contributor(s):

Material type: Article

ArticleLanguage: English Description: 19:187-194Subject(s): LOC classification:

28901

In: Tropical Agriculture Research SeriesSummary: Dos aislamientos de virus obtenidos de Phaseolus vulgaris en el Libano se identificaron como potyvirus con estrecha relacion serologica entre si, con el virus del mosaico del caupi `ojo negro', con BCMV-NY 15 y con el virus del mosaico del frijol adzuki (AzMV). Ambos aislamientos infectaron 6 especies de plantas pertenecientes a 3 familias. Los 2 aislamientos se diferenciaron por su reaccion en 6 lineas IITA-TVu de caupi como tambien en 7 cv. de P. vulgaris. En pruebas ELISA de doble sandwich ambos antigenos virales reaccionaron fuertemente con antisueros del virus del mosaico del caupi `ojo negro', BCMV-NY 15 y AzMV, y debilmente con antisueros del virus del mosaico del caupi transmitido por afidos, BYMV y algunos aislamientos del BCMV. En pruebas de ELISA reciprocas, los antisueros para estos 2 virus reaccionaron fuertemente con el virus del mosaico del caupi `ojo negro' y BCMV- NY 15. Las pruebas de microscopia electronica inmunosorbente confirmaron los resultados obtenidos con ELISA; se presentaron reacciones fuertes cuando ambos aislamientos se probaron con antisueros del virus del mosaico del caupi `ojo negro', BCMV-NY 15 y AzMV. (RA-CIAT)Summary: Two virus isolates obtained from Phaseolus vulgaris in Lebanon were identified as potyviruses with close serological relationship to each other, to blackeye cowpea mosaic virus, to the BCMV-NY 15, and to adzuki bean mosaic virus (AzMV). Both isolates infected 6 plant species belonging to 3 families. The 2 isolates were differentiated from each other by their reaction on 6 IITA-TVu cowpea lines as well as on 7 P. vulgaris cv. In double antibody sandwich ELISA both virus antigens strongly reacted with blackeye cowpea mosaic virus, BCMV-NY 15, and AzMV antisera and weakly with antisera to cowpea aphid-borne mosaic virus, BYMV, and some isolates of BCMV. In reciprocal ELISA tests, antisera to these 2 virus isolates reacted strongly with blackeye cowpea mosaic virus and BCMV-NY 15. Immunosorbent electron microscopy tests confirmed the ELISA results, giving strong reactions when both isolates were tested with antisera to blackeye cowpea mosaic virus, BCMV-NY 15, and AzMV. (AS)

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Holdings
Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode	Item holds
Journal Article	CIAT Library Document collection CINFOS	Document Collection CINFOS	28901 (Browse shelf(Opens below))	c.1	Short Loan		100064259

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Dos aislamientos de virus obtenidos de Phaseolus vulgaris en el Libano se identificaron como potyvirus con estrecha relacion serologica entre si, con el virus del mosaico del caupi `ojo negro', con BCMV-NY 15 y con el virus del mosaico del frijol adzuki (AzMV). Ambos aislamientos infectaron 6 especies de plantas pertenecientes a 3 familias. Los 2 aislamientos se diferenciaron por su reaccion en 6 lineas IITA-TVu de caupi como tambien en 7 cv. de P. vulgaris. En pruebas ELISA de doble sandwich ambos antigenos virales reaccionaron fuertemente con antisueros del virus del mosaico del caupi `ojo negro', BCMV-NY 15 y AzMV, y debilmente con antisueros del virus del mosaico del caupi transmitido por afidos, BYMV y algunos aislamientos del BCMV. En pruebas de ELISA reciprocas, los antisueros para estos 2 virus reaccionaron fuertemente con el virus del mosaico del caupi `ojo negro' y BCMV- NY 15. Las pruebas de microscopia electronica inmunosorbente confirmaron los resultados obtenidos con ELISA; se presentaron reacciones fuertes cuando ambos aislamientos se probaron con antisueros del virus del mosaico del caupi `ojo negro', BCMV-NY 15 y AzMV. (RA-CIAT) spa

Two virus isolates obtained from Phaseolus vulgaris in Lebanon were identified as potyviruses with close serological relationship to each other, to blackeye cowpea mosaic virus, to the BCMV-NY 15, and to adzuki bean mosaic virus (AzMV). Both isolates infected 6 plant species belonging to 3 families. The 2 isolates were differentiated from each other by their reaction on 6 IITA-TVu cowpea lines as well as on 7 P. vulgaris cv. In double antibody sandwich ELISA both virus antigens strongly reacted with blackeye cowpea mosaic virus, BCMV-NY 15, and AzMV antisera and weakly with antisera to cowpea aphid-borne mosaic virus, BYMV, and some isolates of BCMV. In reciprocal ELISA tests, antisera to these 2 virus isolates reacted strongly with blackeye cowpea mosaic virus and BCMV-NY 15. Immunosorbent electron microscopy tests confirmed the ELISA results, giving strong reactions when both isolates were tested with antisera to blackeye cowpea mosaic virus, BCMV-NY 15, and AzMV. (AS) eng