Association between BCMV resistant I gene and eye color of cv. Steuben

Para determinar la relacion entre el gen I y el color del hilo, se seleccionaron una pocas lineas de frijol con hilo amarillo, gris y cafe oscuro, de poblaciones R3 RC5 de un cruzamiento Steuben 4/PI326.418. Algunas de las lineas escogidas eran segregantes para el color del hilo. Las plantas F2 se inocularon con las razas 1 y 15 de BCMV en estado unifoliado y se examinaron para el color del hilo. Todas las plantas de la clase de hilo amarillo fueron susceptibles al BCMV y segregaron unicamente en hilo de color amarillo. Las plantas pertenecientes a las clases gris y cafe segregaron hacia las clases crema, amarillo, gris y cafe. Todas las plantas susceptibles a BCMV produjeron unicamente semilla con hilo color crema y amarillo y todas las plantas resistentes produjeron semilla con hilo gris o cafe. Como solamente las plantas resistentes produjeron unicamente semilla con hilo gris o cafe, el gen I resistente dominante y el gen B cafe-verdoso deben estar estrechamente ligados en la fase de acoplamiento. (CIAT) spa

To determine the relationship between the I gene and eye color, a few bean lines with yellow, grey, and dark brown eye color were selected from cross Steuben 4/PI326.418 BC5 F3 populations. Some of the selected lines were segregating for eye color. F2 plants were inoculated with BCMV races 1 and 15 at the unifoliate stage, and examined for eye color. All the plants from the yellow eye class were susceptible to BCMV and segregated into creamy and yellow eye only. Plants in the grey and brown classes segregated into creamy, yellow, grey, and brown classes. All the BCMV-susceptible plants produced seed only with creamy or yellow eyes and all the resistant plants produced seed with grey or brown eye color. Since only BCMV- resistant plants produced seed with grey or brown eyes, the dominant resistant I gene and greenish brown B gene must be tightly linked in coupling phase. (CIAT) eng