Translatable mRNA changes in ethylene induced abscission zones of Phaseolus vulgaris (Red Kidney)

By:

KELLY, P

Contributor(s):

Material type: Article

ArticleLanguage: English Description: 10(1):11-16Subject(s): LOC classification:

29950

In: Plant, Cell and EnvironmentSummary: Se programo el sistema de translacion del germen de trigo con ARN soluble extraido de zonas de abscision foliar de Phaseolus vulgaris. Estos extractos se tomaron en diferentes periodos despues de la induccion de la abscision. Solo estuvo presente un producto de translacion con un p. mol. de 42 kilodaltones despues de este tratamiento, aunque otras 3 especies de 32, 27 y 17 kilodaltones aumentaron sustancialmente. La isoenzima de celulosa con un PI de 9.5 no pudo ser identificada finalmente entre los productos, si bien la proteina de 32 kilodaltones es probablemente la quitinasa. La comparacion de la zona de abscision del ARN traducible con la de los tejidos adyacentes del peciolo y tallo indicaron que la proteina de 17 kilodaltones se desarrollo en todos estos sitios. Se encontraron las bandas de 42, 32 y 27 kilodaltones predominantemente en la zona de abscision y en el peciolo. (RA-CIAT)Summary: The wheat germ translation system was programmed with soluble RNA extracted from foliar abscission zones of Phaseolus vulgaris. These extracts were taken at various times after the induction of abscission. A translation product with a mol. wt. of 42 kilodaltons was only present after this treatment, though 3 other species of 32, 27, and 17 kilodaltons increased substantially. The isozyme of cellulase with a pI of 9.5 could not be conclusively identified among the products, though the 32 kilodaltons protein is probably chitinase. Comparison of the abscission zone translatable RNA with that from adjacent petiole and stem tissues showed the 17 kilodaltons protein developed in all these locations. The 42, 32, and 27 kilodaltons bands were found predominantly in the abscission zone and petiole. (AS)

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Holdings
Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode	Item holds
Journal Article	CIAT Library Document collection CINFOS	Document Collection CINFOS	29950 (Browse shelf(Opens below))	c.1	Short Loan		100052844

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Se programo el sistema de translacion del germen de trigo con ARN soluble extraido de zonas de abscision foliar de Phaseolus vulgaris. Estos extractos se tomaron en diferentes periodos despues de la induccion de la abscision. Solo estuvo presente un producto de translacion con un p. mol. de 42 kilodaltones despues de este tratamiento, aunque otras 3 especies de 32, 27 y 17 kilodaltones aumentaron sustancialmente. La isoenzima de celulosa con un PI de 9.5 no pudo ser identificada finalmente entre los productos, si bien la proteina de 32 kilodaltones es probablemente la quitinasa. La comparacion de la zona de abscision del ARN traducible con la de los tejidos adyacentes del peciolo y tallo indicaron que la proteina de 17 kilodaltones se desarrollo en todos estos sitios. Se encontraron las bandas de 42, 32 y 27 kilodaltones predominantemente en la zona de abscision y en el peciolo. (RA-CIAT)

The wheat germ translation system was programmed with soluble RNA extracted from foliar abscission zones of Phaseolus vulgaris. These extracts were taken at various times after the induction of abscission. A translation product with a mol. wt. of 42 kilodaltons was only present after this treatment, though 3 other species of 32, 27, and 17 kilodaltons increased substantially. The isozyme of cellulase with a pI of 9.5 could not be conclusively identified among the products, though the 32 kilodaltons protein is probably chitinase. Comparison of the abscission zone translatable RNA with that from adjacent petiole and stem tissues showed the 17 kilodaltons protein developed in all these locations. The 42, 32, and 27 kilodaltons bands were found predominantly in the abscission zone and petiole. (AS)