Morphogenetic effects of 2,4-dichlorophenoxyacetic acid on pinto bean (Phaseolus vulgaris L.) leaf explants in vitro

By:

SAUNDERS, J.W

Contributor(s):

Material type: Article

ArticleLanguage: English Description: 6:46-49Subject(s): LOC classification:

31713

In: Plant Cell ReportsSummary: Se produjeron estructuras de raices, callos y/o globulos de hojas primarias y explantos de cotiledon distal de frijol pinto (Phaseolus vulgaris cv. UI 114) cultivado en medio semisolido Murashige-Skoog con un amplio rango de concn. de 2,4-D (0.01 a 80 mg/litro) con 0 o 1 mg de cinetina/litro. Los explantos enraizaron a menores concn. de 2,4-D que a aquellas que favorecieron la formacion de globulos en callos, aunque a menudo se desarrollaron raices, callos y globulos a partir de los mismos explantos. Cuando los callos se iniciaron en un medio con 3 o mas mg de 2,4-D/litro y se subcultivaron en liquido Murashige-Skoog + 30 mg de 2,4-D/litro se desarrollaron estructuras globulares opacas aisladas y de color verde. Estas estructuras se multiplicaron con un peso fresco duplicando el tiempo de 8-9 dias en Murashige-Skoog + 30 mg de 2,4- D/litro. Aunque el comportamiento multiplicativo y el color opaco evocaron embrioides reportados en otras especies, no se observaron cotiledones ni raices. (RA-CIAT)Summary: Roots, callus and/or globular structures were produced on primary leaf and distal cotyledon explants of pinto bean (Phaseolus vulgaris cv. UI 114) cultured on semisolid Murashige-Skoog (MS) medium with a wide range of 2,4-D concn. (0.01 to 80 mg/liter with either 0 or 1 mg/liter kinetin. Explants rooted at lower 2,4-D concn. than at those favoring globule formation on callus, although roots, callus and globules often developed from the same explant. Isolated opaque green globular structures developed when callus initiated on media with 3 or more mg/liter 2,4-D was subcultured in liquid MS + 30 mg/liter 2,4-D. These structures multiplied with a fresh wt. doubling time of 8-9 days in MS + 30 mg/liter 2,4-D. Although this multiplicative behavior and opaque color were reminiscent of embryoids reported for other species, no cotyledons or roots were seen. (AS)

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Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode	Item holds
Journal Article	CIAT Library Document collection CINFOS	Document Collection CINFOS	31713 (Browse shelf(Opens below))	c.1	Short Loan		100042623

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Previous	31710 Expression of circaseptan and circannual rhythmicity in the imbibition of dry stored bean seeds	31711 The effect of temperature on the occurrence of O2 and CO2 insensitive photosynthesis in field grown plants	31712 Effects of sulfur dioxide exposure on the development of common blight in field-grown red kidney beans	31713 Morphogenetic effects of 2,4-dichlorophenoxyacetic acid on pinto bean (Phaseolus vulgaris L.) leaf explants in vitro	31714 Field measurement of white mold effects upon dry beans with genetic resistance or upright plant architecture	31715 Strategies for chemical control of snap bean rust in Florida and their compatibility with Canadian residue tolerances	31716 Effects of herbicides on root rot of pinto bean, weeds, and two soilborne fungi	Next

Se produjeron estructuras de raices, callos y/o globulos de hojas primarias y explantos de cotiledon distal de frijol pinto (Phaseolus vulgaris cv. UI 114) cultivado en medio semisolido Murashige-Skoog con un amplio rango de concn. de 2,4-D (0.01 a 80 mg/litro) con 0 o 1 mg de cinetina/litro. Los explantos enraizaron a menores concn. de 2,4-D que a aquellas que favorecieron la formacion de globulos en callos, aunque a menudo se desarrollaron raices, callos y globulos a partir de los mismos explantos. Cuando los callos se iniciaron en un medio con 3 o mas mg de 2,4-D/litro y se subcultivaron en liquido Murashige-Skoog + 30 mg de 2,4-D/litro se desarrollaron estructuras globulares opacas aisladas y de color verde. Estas estructuras se multiplicaron con un peso fresco duplicando el tiempo de 8-9 dias en Murashige-Skoog + 30 mg de 2,4- D/litro. Aunque el comportamiento multiplicativo y el color opaco evocaron embrioides reportados en otras especies, no se observaron cotiledones ni raices. (RA-CIAT) spa

Roots, callus and/or globular structures were produced on primary leaf and distal cotyledon explants of pinto bean (Phaseolus vulgaris cv. UI 114) cultured on semisolid Murashige-Skoog (MS) medium with a wide range of 2,4-D concn. (0.01 to 80 mg/liter with either 0 or 1 mg/liter kinetin. Explants rooted at lower 2,4-D concn. than at those favoring globule formation on callus, although roots, callus and globules often developed from the same explant. Isolated opaque green globular structures developed when callus initiated on media with 3 or more mg/liter 2,4-D was subcultured in liquid MS + 30 mg/liter 2,4-D. These structures multiplied with a fresh wt. doubling time of 8-9 days in MS + 30 mg/liter 2,4-D. Although this multiplicative behavior and opaque color were reminiscent of embryoids reported for other species, no cotyledons or roots were seen. (AS) eng