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Differential changes in the synthesis and steady-state levels of thylakoid proteins during bean leaf senescence

By: Contributor(s): Material type: ArticleArticleLanguage: English Description: 9(4):343-353Subject(s): LOC classification:
  • 31812
 In: Plant Molecular BiologySummary: Expt. de deteccion de impulso intermitente con hojas intactas de frijol indicaron degradacion rapida de 32 proteinas kD, consistentes con su tasa de rapidez de renovacion conocida. La degradacion dependio de la luz y resulto inhibida por DCMU; la cinetica de la degradacion fue similar tanto para las membranas jovenes como para las senescentes. En geles de Coomassie tenidos, las proteinas dominantes para todas las edades de las membranas fueron el centro de reaccion kD 68 del polipeptido del fotosistema I y las subunidades alfa y beta de ATPasa y LHCP. El analisis por absorcion en papel de filtro indico que los citocromas f y b6 se disminuyen selectivamente durante la senescencia. Se han interpretado datos que indican que las interrupciones de translacion tanto en los compartimientos citoplasmicos como en los cloroplasticos pueden contribuir a la disminucion del transporte de electrones fotosinteticos en la hoja senescente. (RA (extracto)- CIAT)Summary: Pulse-chase expt. with intact bean leaves indicated rapid degradation of the 32 kD protein, consistent with its known rapid turnover rate. Degradation was light-dependent and inhibited by DCMU; degradation kinetics were similar for young and senescent membranes. In Coomassie-stained gels, the 68 kD reaction center polypeptide of photosystem I, the alpha and beta subunits of ATPase and the LHCP were the dominant proteins for all ages of membranes. Western blot analysis indicated that cytochrome f and cytochrome b6 are selectively depleted during senescence. Data have been interpreted as indicating that translational disruptions in both the cytoplasmic and chloroplastic compartments may contribute to the decline in photosynthetic electron transport in the senescing leaf. (AS (extract))
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Expt. de deteccion de impulso intermitente con hojas intactas de frijol indicaron degradacion rapida de 32 proteinas kD, consistentes con su tasa de rapidez de renovacion conocida. La degradacion dependio de la luz y resulto inhibida por DCMU; la cinetica de la degradacion fue similar tanto para las membranas jovenes como para las senescentes. En geles de Coomassie tenidos, las proteinas dominantes para todas las edades de las membranas fueron el centro de reaccion kD 68 del polipeptido del fotosistema I y las subunidades alfa y beta de ATPasa y LHCP. El analisis por absorcion en papel de filtro indico que los citocromas f y b6 se disminuyen selectivamente durante la senescencia. Se han interpretado datos que indican que las interrupciones de translacion tanto en los compartimientos citoplasmicos como en los cloroplasticos pueden contribuir a la disminucion del transporte de electrones fotosinteticos en la hoja senescente. (RA (extracto)- CIAT) spa

Pulse-chase expt. with intact bean leaves indicated rapid degradation of the 32 kD protein, consistent with its known rapid turnover rate. Degradation was light-dependent and inhibited by DCMU; degradation kinetics were similar for young and senescent membranes. In Coomassie-stained gels, the 68 kD reaction center polypeptide of photosystem I, the alpha and beta subunits of ATPase and the LHCP were the dominant proteins for all ages of membranes. Western blot analysis indicated that cytochrome f and cytochrome b6 are selectively depleted during senescence. Data have been interpreted as indicating that translational disruptions in both the cytoplasmic and chloroplastic compartments may contribute to the decline in photosynthetic electron transport in the senescing leaf. (AS (extract)) eng

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