Quantitation of indoleacetic acid conjugates in bean seeds by direct tissue hydrolysis

By:

BIALEK, K

Contributor(s):

COHEN, J.D

Material type: Article

ArticleLanguage: English Description: 90:398-400Subject(s): LOC classification:

37449

Online resources:

Click here to access online

In: Plant PhysiologySummary: El analisis por espectrometria de masas-seguimiento de iones seleccionados-cromotografia de gases, utilizando ((13)C6) AIA como estandar interno, proporciona un medio efectivo para cuantificar el AIA liberado durante la hidrolisis basica directa e intensa de polvo de semilla de frijol, siempre y cuando se tenga la precaucion de excluir el oxigeno de la ampolla de reaccion. La hidrolisis directa de polvo de semilla revelo que la principal porcion de conjugados de AIA amidas en semillas de frijol no se puede extraer con acetona acuosa, el solvente comunmente usado para extraer conjugados de AIA de semillas y de otros tejidos vegetales. Es posible utilizar hidrolisis basica intensa de tejido vegetal para proporcionar nueva informacion sobre el contenido de AIA. (RA-CIAT)Summary: Gas chromatography-selected ion monitoring-mass spectral analysis using (13C6)indole-3-acetic acid (IAA) as an internal standard provides an effective means for quantitation of IAA liberated during direct strong basic hydrolysis of bean (Phaseolus vulgaris L.) seed powder, provided that extra precautions are undertaken to exclude oxygen from the reaction vial. Direct seed powder hydrolysis revealed that the major portion of amide IAA conjugates in bean seeds are not extractable by aqueous acetone, the solvent used commonly for IAA conjugate extraction from seeds and other plant tissues. Strong basic hydrolysis of plant tissue can be used to provide new information on IAA content. (AS)

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Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode	Item holds
Online Document	CIAT Library Web	Electronic Document	37449 (Browse shelf(Opens below))		Not For Loan (Restricted Access)
Journal Article	CIAT Library Document collection CINFOS	Document Collection CINFOS	37449 (Browse shelf(Opens below))	c.1	Short Loan		100056817

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Previous	37367 Comparison of leaf and pod disease reactions of beans (Phaseolus vulgaris L.) inoculated by different methods with strains of Xanthomonas campestris pv. Phaseoli (Smith) Dye	37368 Variation of the storage protein phaseolin in common bean (Phaseolus vulgaris L.) from the Mediterranean area	37395 Cold tolerance in beans (Phaseolus spp.) as analyzed by their exotherms	37449 Quantitation of indoleacetic acid conjugates in bean seeds by direct tissue hydrolysis	37450 Use of per-C-deuterated myo-inositol for study of cell wall synthesis in germinating beans	37456 Comparison of isozyme and virulence diversity patterns in the bean rust fungus Uromyces appendiculatus	37464 Genetic and cultural control of Fusarium root rot in bush snap beans	Next

El analisis por espectrometria de masas-seguimiento de iones seleccionados-cromotografia de gases, utilizando ((13)C6) AIA como estandar interno, proporciona un medio efectivo para cuantificar el AIA liberado durante la hidrolisis basica directa e intensa de polvo de semilla de frijol, siempre y cuando se tenga la precaucion de excluir el oxigeno de la ampolla de reaccion. La hidrolisis directa de polvo de semilla revelo que la principal porcion de conjugados de AIA amidas en semillas de frijol no se puede extraer con acetona acuosa, el solvente comunmente usado para extraer conjugados de AIA de semillas y de otros tejidos vegetales. Es posible utilizar hidrolisis basica intensa de tejido vegetal para proporcionar nueva informacion sobre el contenido de AIA. (RA-CIAT) spa

Gas chromatography-selected ion monitoring-mass spectral analysis using (13C6)indole-3-acetic acid (IAA) as an internal standard provides an effective means for quantitation of IAA liberated during direct strong basic hydrolysis of bean (Phaseolus vulgaris L.) seed powder, provided that extra precautions are undertaken to exclude oxygen from the reaction vial. Direct seed powder hydrolysis revealed that the major portion of amide IAA conjugates in bean seeds are not extractable by aqueous acetone, the solvent used commonly for IAA conjugate extraction from seeds and other plant tissues. Strong basic hydrolysis of plant tissue can be used to provide new information on IAA content. (AS) eng