Extended map for the phaseolin linkage group of Phaseolus vulgaris L

By:

Vallejos, C.E

Contributor(s):

Chase, C.D

Material type: Article

ArticleLanguage: English Description: 82(3):353-357Subject(s): LOC classification:

41165

In: Theoretical and Applied Genetics (Germany)Summary: Se analizaron la relacion del vinculo de 11 proteinas de la semilla de frijol (incluyendo faseolina), 9 loci de la enzima y el locus de P en progenies de retrocruzamientos y F2 mediante el uso del paquete de software Mapmaker. Las progenies se obtuvieron mediante el cruce de la linea mejorada XR-235-1 y el cv. Calima. Se detectaron diferencias de alelos para los loci de proteina de semilla con electroforesis de geles de poliacrilamida-DSS y aquellos para los loci de la enzima con electroforesis de geles de almidon y colorantes de actividad. El color de la testa de la semilla del Calima es un modelo moteado rojo/beige y el de XR-235-1 es blanco. Se siguio la segregacion en el locus P registrando el fenotipo del BC1S1 y la semilla de F3. Un grupo del vinculo que comprende ca. 90 cM se detecto con el orden siguiente de genes: Est- 2 - 11 - pha - 8 -(Spe/Spg) - 24- P - 9 -(Spa/Spb)-16 - Spba - 22 - Mdh-1. Ademas, se detecto otro grupo del vinculo: (Spd/spf/sph) - 5 -Spca. En consecuencia, parece que las proteinas de semilla se organizan en los conglomerados en el genoma de frijol. (RA-CIAT)Summary: The linkage relationship of 11 bean (Phaseolus vulgaris) seed proteins (including phaseolin), 9 enzyme loci, and the P locus were analyzed in backcross and F2 progenies by use of the software package Mapmaker. The progenies were obtained by crossing the breeding line XR-235-1 and the cultivar Calima. Allelic differences for seed protein loci were detected with SDS-PAGE and those for enzyme loci with starch gel electrophoresis and activity stains. The seed coat color of Calima is a red/beige mottled pattern and that of XR-235-1 is white. Segregation at the P locus was followed by recording the phenotype of the BC1S1 and F3 seed. A linkage group comprising ca. 90 cM was detected with the following gene order: Est-2 - 11 - pha - 8 -(Spe/Spg) - 24- P - 9 -(Spa/Spb)-16 - Spba - 22 - Mdh-1. In addition, another linkage group was detected: (Spd/spf/sph) - 5 - Spca. Therefore, the seed proteins appear to be organized in clusters in the bean genome. (AS)

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Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode	Item holds
Journal Article	CIAT Library Document collection CINFOS	Document Collection CINFOS	41165 (Browse shelf(Opens below))	c.1	Short Loan		100059670

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Previous	41162 Contribuciones agrosociologicas a las investigaciones sobre yuca y arroz en la Republica Dominicana: informe final de la investigacion agrosociologica sobre yuca y arroz 1981-1985	41163 Los clones de yuca mas importantes en Cuba	41164 Changes in cell wall polysaccharides during the growth of Phaseolus vulgaris leaves	41165 Extended map for the phaseolin linkage group of Phaseolus vulgaris L	41166 Distribution of cadmium and induced Cd-binding proteins in roots, stems and leaves of Phaseolus vulgaris	41167 Effect of temperature on net CO2 assimilation and photosystem II quantum yield of electron transfer of French bean (Phaseolus vulgaris L.) leaves during drought stress	41168 Composition and nutritive value of dry edible beans: commercial and world food relief applications	Next

Se analizaron la relacion del vinculo de 11 proteinas de la semilla de frijol (incluyendo faseolina), 9 loci de la enzima y el locus de P en progenies de retrocruzamientos y F2 mediante el uso del paquete de software Mapmaker. Las progenies se obtuvieron mediante el cruce de la linea mejorada XR-235-1 y el cv. Calima. Se detectaron diferencias de alelos para los loci de proteina de semilla con electroforesis de geles de poliacrilamida-DSS y aquellos para los loci de la enzima con electroforesis de geles de almidon y colorantes de actividad. El color de la testa de la semilla del Calima es un modelo moteado rojo/beige y el de XR-235-1 es blanco. Se siguio la segregacion en el locus P registrando el fenotipo del BC1S1 y la semilla de F3. Un grupo del vinculo que comprende ca. 90 cM se detecto con el orden siguiente de genes: Est- 2 - 11 - pha - 8 -(Spe/Spg) - 24- P - 9 -(Spa/Spb)-16 - Spba - 22 - Mdh-1. Ademas, se detecto otro grupo del vinculo: (Spd/spf/sph) - 5 -Spca. En consecuencia, parece que las proteinas de semilla se organizan en los conglomerados en el genoma de frijol. (RA-CIAT) spa

The linkage relationship of 11 bean (Phaseolus vulgaris) seed proteins (including phaseolin), 9 enzyme loci, and the P locus were analyzed in backcross and F2 progenies by use of the software package Mapmaker. The progenies were obtained by crossing the breeding line XR-235-1 and the cultivar Calima. Allelic differences for seed protein loci were detected with SDS-PAGE and those for enzyme loci with starch gel electrophoresis and activity stains. The seed coat color of Calima is a red/beige mottled pattern and that of XR-235-1 is white. Segregation at the P locus was followed by recording the phenotype of the BC1S1 and F3 seed. A linkage group comprising ca. 90 cM was detected with the following gene order: Est-2 - 11 - pha - 8 -(Spe/Spg) - 24- P - 9 -(Spa/Spb)-16 - Spba - 22 - Mdh-1. In addition, another linkage group was detected: (Spd/spf/sph) - 5 - Spca. Therefore, the seed proteins appear to be organized in clusters in the bean genome. (AS) eng