Characterization of cassava common mosaic virus and a defective RNA species
Material type:
ArticleLanguage: English Description: 77(3):525-530Subject(s): - Manihot esculenta
- Cassava mosaic virus
- Nucleotide sequence
- RNA
- Manihot esculenta
- Virus del mosaico de la casava
- Secuencia nucleotídica
- ARN
- Thompson
- Cassava
- Yuca
- CIAT Autor
- RJ
- Electronic documents
- Documentos electrónicos
- Genética vegetal y fitomejoramiento
- Plant genetics and breeding
- Articles in Refereed Journals
| Item type | Current library | Collection | Call number | Copy number | Status | Date due | Barcode | Item holds | |
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Online Document
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CIAT Library Web | Electronic Document | Not For Loan (Restricted Access) | ||||||
Journal Article
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CIAT Library Journal Collection | Journal Collection | c.1 | Not For Loan (Restricted Access) |
The genome of cassava common mosaic potexvirus (CsCMV) has been sequenced from cDNA clones and consists of 6376 nucleotides (nt). A 76 nt untranslated region (UTR) at the 5' terminus was followed by ORF1 which potentially encodes a protein of 1449 amino acids (aa). ORFs 2, 3, and 4 were predicted to encode proteins of 231, 112 and 97 aa, respectively. ORF5 potentially encodes a 229 aa protein of 25 kDa that is similar to the coat proteins of other potexviruses. The 3'-terminal UTR of 114 nt was followed by a poly(A) tail. The genomic organization of the CsCMV genome is similar to that of other potexviruses. A cDNA clone that was apparently obtained from a defective RNA species contained both the 5' and 3' UTRs and an ORF that potentially encodes the first 263 aa of ORF1 and the last 33 aa of the coat protein. Defective RNA species were found both in purified preparations of the virus and in total nucleic acid isolated from CsCMV-infected plants eng