Method for the quantification of acid production by plants in gel

By:

Logan, Katherine A.B

Contributor(s):

Thomas, Richard J

Material type: Article

ArticleLanguage: English Description: 28(17-18):1633-1641Subject(s): LOC classification:

62791

In: Communications in Soil Science and Plant Analysis (USA)Summary: Hydrogen (H+) and hydroxyl ion (OH-) production by Brachiaria humidicola is quantified using a method in which the plants are grown in soil then transferred to agar gel for 24 h. The amount of H+ and OH- produced was calculated from the pH of the melted gel and the gels' buffer curve. Values were obtained for plants of different ages and with nitrogen (N) supplied in the gel as nitrate (NO3-), ammonium (NH4+), or ammonium nitrate (NH4NO3) and compared with data calculated using the sum of H+ changes in differently colored zones of the gel. Daily H+ and OH- production increased with plant age and total dry matter for the NH4+- and NO3--fed plants, respectively. By integrating the data over time, a value of 0.33 mmol H+ plant-1 was obtained for the total H+ production over 62 days. The proposed method was sufficiently rapid and versatile to allow the comparison between plant species or cultivars, which were grown using a variety of nutrient supplies. This procedure may indicate how acid production affects plant nutrient acquisition and aid the prediction of soil acidification by different plant species or cultivars. (RA)

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Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode	Item holds
Journal Article	CIAT Library Document collection CINFOS	Document Collection CINFOS	62791 (Browse shelf(Opens below))	c.1	Short Loan		100071962

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Previous	62788 Carbon and nitrogen dynamics in ageing earthworm casts in grasslands of the eastern plains of Colombia	62789 Phosphorus acquisition and cycling in crop and pasture systems in low fertility tropical soils	62790 Lignin and carbohydrate alteration in particle-size separates of an oxisol under tropical pastures following native savanna	62791 Method for the quantification of acid production by plants in gel	62795 CIAT's strategic research for sustainable land management on the steep hillsides of Latin America	62796 The role of forage grasses and legumes in maintaining the productivity of acid soils in Latin America	62798 The use of carbon isotope ratios to evaluate legume contribution to soil enhancement in tropical pastures	Next

Hydrogen (H+) and hydroxyl ion (OH-) production by Brachiaria humidicola is quantified using a method in which the plants are grown in soil then transferred to agar gel for 24 h. The amount of H+ and OH- produced was calculated from the pH of the melted gel and the gels' buffer curve. Values were obtained for plants of different ages and with nitrogen (N) supplied in the gel as nitrate (NO3-), ammonium (NH4+), or ammonium nitrate (NH4NO3) and compared with data calculated using the sum of H+ changes in differently colored zones of the gel. Daily H+ and OH- production increased with plant age and total dry matter for the NH4+- and NO3--fed plants, respectively. By integrating the data over time, a value of 0.33 mmol H+ plant-1 was obtained for the total H+ production over 62 days. The proposed method was sufficiently rapid and versatile to allow the comparison between plant species or cultivars, which were grown using a variety of nutrient supplies. This procedure may indicate how acid production affects plant nutrient acquisition and aid the prediction of soil acidification by different plant species or cultivars. (RA) eng