Acid PAGE procedure for detection of phaseolins in field beans (Phaseolus vulgaris L.)

By:

Hussain, A

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Material type: Article

ArticleLanguage: English Description: 44(1-2):1-3Subject(s): LOC classification:

37366

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In: EuphyticaSummary: Se describe un procedimiento basado en electroforesis en geles de poliacrilamida (EGPA) acida. La resolucion de franjas de faseolina es relativamente superior a la obtenida con el sistema basico de EGPA. El procedimiento discrimina los tipos de faseolina S, T, C, M y B; los tipos S, T y C ya se habian reportado anteriormente mientras que los tipos M y B se identificaron en el presente estudio. (RA-CIAT)Summary: A procedure based on acid polyacrylamide gel electrophoresis (acid PAGE) has been described. Resolution of phaseolin bands appears to be relatively superior to that obtained with basic PAGE system. The procedure discriminates S, T, C, M and B types of phaseolins; S, T and C have been reported previously while M and B types were identified in the present study. (AS)

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Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode	Item holds
Online Document	CIAT Library Web	Electronic Document	37366 (Browse shelf(Opens below))		Not For Loan (Restricted Access)
Journal Article	CIAT Library Document collection CINFOS	Document Collection CINFOS	37366 (Browse shelf(Opens below))	c.1	Short Loan		100056765

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Previous	37355 Isolation and partial characterization of a geminivirus causing bean dwarf mosaic	37358 Factors determining the efficacy of chemical control of white mold in white bean	37363 (15)N-determined dinitrogen fixation potential of genetically diverse bean lines (Phaseolus vulgaris L.)	37366 Acid PAGE procedure for detection of phaseolins in field beans (Phaseolus vulgaris L.)	37367 Comparison of leaf and pod disease reactions of beans (Phaseolus vulgaris L.) inoculated by different methods with strains of Xanthomonas campestris pv. Phaseoli (Smith) Dye	37368 Variation of the storage protein phaseolin in common bean (Phaseolus vulgaris L.) from the Mediterranean area	37395 Cold tolerance in beans (Phaseolus spp.) as analyzed by their exotherms	Next

Se describe un procedimiento basado en electroforesis en geles de poliacrilamida (EGPA) acida. La resolucion de franjas de faseolina es relativamente superior a la obtenida con el sistema basico de EGPA. El procedimiento discrimina los tipos de faseolina S, T, C, M y B; los tipos S, T y C ya se habian reportado anteriormente mientras que los tipos M y B se identificaron en el presente estudio. (RA-CIAT) spa

A procedure based on acid polyacrylamide gel electrophoresis (acid PAGE) has been described. Resolution of phaseolin bands appears to be relatively superior to that obtained with basic PAGE system. The procedure discriminates S, T, C, M and B types of phaseolins; S, T and C have been reported previously while M and B types were identified in the present study. (AS) eng