TY  - SER
AU  - LEBEDEV, N.N.
AU  - Pakshina, Y.V.
AU  - Shiffel, P.
AU  - KRASNOVSKII, A.A.
TI  - Spectral effects of the interaction of hydrogen ions with the chloroplast membrane
AV  - 41172 
KW  - Phaseolus vulgaris
KW  - CHLOROPHYLL
KW  - Chloroplasts
KW  - Photosynthesis
KW  - Light
KW  - pH
KW  - Biochemistry
KW  - Climatic requirements
KW  - Cultivation
KW  - Physiology
KW  - PLANT PHYSIOLOGICAL PROCESSES
KW  - Plant physiology
KW  - PLANT PIGMENTS
KW  - CLOROFILA
KW  - CLOROPLASTOS
KW  - Fotosintesis
KW  - Luz
KW  - BIOQUIMICA
KW  - Journal articles
KW  - Artículos en revistas
KW  - Frijol
KW  - Beans
KW  - Journal article
N2  - Se investigo la influencia de la exposicion a la accion breve de una concn. alta de los iones de H en la absorcion de bajas temp. y el espectro de fluorescencia de los cloroplastos. Se indica que tal exposicion produce un cambio reversible de onda corta en las bandas de las formas de la clorofila a y b que forman parte del complejo colector de luz (absorcion max. de 647, 660, 676 nm) y tambien la banda ancha de onda larga (absorcion en la region 710 y fluorescencia a 735 nm). Un cambio de la onda corta en las bandas de absorcion de los carotenoides (en la region 480-520 nm) se observa simultaneamente similar al cambio electrocromico inducido por la luz pero de signo opuesto. Se ha establecido que la interaccion con iones de H de las formas de onda larga de la clorofila a (max. absorcion de 682, 690 y 696 nm y max. fluorescencia a 685 y 696 nm) especifica para los fotosistemas II y I conducen a su decoloracion irreversible. La forma de onda corta, posiblemente monomera, de la clorofila con max. absorcion a 666 nm no es sensible a la exposicion breve a los iones de H. (RA-CIAT); The authors have investigated the influence of exposure to the brief action of a high concentration of hydrogen ions on the low temperature absorption and fluorescence spectra of the chloroplasts. It is shown that such exposure produces a reversible shortwave shift in the bands of the forms of chlorophyll a and b forming part of the light-collecting complex (absorption maxima 647, 660, 676 nm) and also the longwave wide band (absorption in the region 710 and fluorescence at 735 nm). A shortwave shift in the absorption bands of the carotenoids (in the region 480-520 nm) is simultaneously observed similar to the photo-induced electrochromic shift but of opposite sign. It has been established that the interaction with hydrogen ions of the longwave forms of chlorophyll a (absorption maxima 682, 690 and 696 nm and fluorescence maxima at 685 and 696 nm) specific for the photosystems II and I leads to their irreversible bleaching. It is shown that the shortwave, possibly monomer, form of chlorophyll with absorption maximum at 666 nm is not sensitive to brief exposure to hydrogen ions. (AS)
ER  -