cDNA cloning, structure, and expression of a novel pathogenesis- related protein in bean

By:

Sharma, Y.K

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Material type: Article

ArticleLanguage: English Description: 5(1):89-95Subject(s): LOC classification:

41785

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In: Molecular Plant-Microbe Interactions (USA)Summary: We have isolated cDNA clones spanning the full length of transcript (PvPR3) encoding a novel pathogenesis-related (PR) protein in bean (Phaseolus vulgaris). The PvPR3 transcript accumulates gradually over 24 h in elicitor-treated cell suspensions. This pattern of expression is distinct from those of previously reported elicitor-induced transcripts in bean. Specifically, transcripts encoding 2 recently described acidic bean PR proteins, phenylpropanoid pathway enzymes, accumulate to maximal levels by 4-8 h while hydroxyproline-rich glycoprotein mRNA accumulation is delayed by several h. The PvPR3 mRNA also accumulates after wounding of hypocotyls with kinetics comparable to those of mRNAs encoding phenylpropanoid pathway mRNAs. PvPR3 appears to exist as a single gene within a family of approx. 15 related genes in the bean genome. The PvPR3 protein deduced from the cDNA sequences (14,950 Da, pl = 10.0) lacks a putative signal peptide suggesting a cytosolic localization. Amino acid sequence comparisons with databases revealed that PvPR3 represents a new class of PR proteins without significant sequence homology to previously characterized PR proteins or other proteins. (AS)

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Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode	Item holds
Online Document	CIAT Library Web	Electronic Document	41785 (Browse shelf(Opens below))		Not For Loan (Restricted Access)
Journal Article	CIAT Library Document collection CINFOS	Document Collection CINFOS	41785 (Browse shelf(Opens below))	c.1	Short Loan		100065172

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We have isolated cDNA clones spanning the full length of transcript (PvPR3) encoding a novel pathogenesis-related (PR) protein in bean (Phaseolus vulgaris). The PvPR3 transcript accumulates gradually over 24 h in elicitor-treated cell suspensions. This pattern of expression is distinct from those of previously reported elicitor-induced transcripts in bean. Specifically, transcripts encoding 2 recently described acidic bean PR proteins, phenylpropanoid pathway enzymes, accumulate to maximal levels by 4-8 h while hydroxyproline-rich glycoprotein mRNA accumulation is delayed by several h. The PvPR3 mRNA also accumulates after wounding of hypocotyls with kinetics comparable to those of mRNAs encoding phenylpropanoid pathway mRNAs. PvPR3 appears to exist as a single gene within a family of approx. 15 related genes in the bean genome. The PvPR3 protein deduced from the cDNA sequences (14,950 Da, pl = 10.0) lacks a putative signal peptide suggesting a cytosolic localization. Amino acid sequence comparisons with databases revealed that PvPR3 represents a new class of PR proteins without significant sequence homology to previously characterized PR proteins or other proteins. (AS) eng