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An automated enzymic assay for determining the cyanide content of cassava (Manihot esculenta Crantz) and cassava products

By: Contributor(s): Material type: ArticleArticleLanguage: English Description: 35(4):426-436Subject(s): LOC classification:
  • 22096
 In: Journal of the Science of Food and AgricultureSummary: Se describe un metodo enzimatico automatizado para el analisis del cianuro en yuca y productos de la yuca. Se pueden manejar facilmente hasta 300 analisis/dia. Un amplio rango de cianuros (0.4- 4.0 microgramos HCN/ml), libres (no glucosidicos) o ligados (glucosidos sustituidos con cianuro), pueden ser determinados en las soluciones extraidas. El limite superior de deteccion del cianuro ligado puede ser aumentado 3 veces facilmente, cuando la determinacion se hace en condiciones de hidrolisis parcial. Dos metodos enzimaticos, uno manual y otro automatizado, concordaron bien en el contenido de cianuro en muestras de hojas y raices peladas almacenadas. (RA-CIAT)Summary: An automated enzymic method for the analysis of cyanide in cassava and cassava products is described. A total of 300 analyses/day can be handled easily. A wide range of free (nonglycosidic) or bound (cyano-substituted glycosides) cyanides (0.4-4.0 micrograms HCN/ml) can be assayed in the extracted solutions. The upper limit of detection for the bound cyanide can easily be increased by another threefold by assaying it under partial hydrolysis conditions. Two enzymic methods, one manual and one automated on Technicon's Auto Analyzer, agreed well for the free and the bound cyanide in the leaf and the peeled storage root samples. (AS)
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Journal Article Journal Article CIAT Library Document collection CINFOS Document Collection CINFOS 22096 (Browse shelf(Opens below)) c.1 Short Loan 100054570
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Se describe un metodo enzimatico automatizado para el analisis del cianuro en yuca y productos de la yuca. Se pueden manejar facilmente hasta 300 analisis/dia. Un amplio rango de cianuros (0.4- 4.0 microgramos HCN/ml), libres (no glucosidicos) o ligados (glucosidos sustituidos con cianuro), pueden ser determinados en las soluciones extraidas. El limite superior de deteccion del cianuro ligado puede ser aumentado 3 veces facilmente, cuando la determinacion se hace en condiciones de hidrolisis parcial. Dos metodos enzimaticos, uno manual y otro automatizado, concordaron bien en el contenido de cianuro en muestras de hojas y raices peladas almacenadas. (RA-CIAT) spa

An automated enzymic method for the analysis of cyanide in cassava and cassava products is described. A total of 300 analyses/day can be handled easily. A wide range of free (nonglycosidic) or bound (cyano-substituted glycosides) cyanides (0.4-4.0 micrograms HCN/ml) can be assayed in the extracted solutions. The upper limit of detection for the bound cyanide can easily be increased by another threefold by assaying it under partial hydrolysis conditions. Two enzymic methods, one manual and one automated on Technicon's Auto Analyzer, agreed well for the free and the bound cyanide in the leaf and the peeled storage root samples. (AS) eng

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