Cultivo de protoplastos de yuca y Stylosanthes
Material type:
TextLanguage: Spanish Series: Seminarios internos SE-5-85Publication details: Cali, CO Centro Internacional de Agricultura Tropical (CIAT) 1985Description: 5 pSubject(s): LOC classification: - 35334
| Item type | Current library | Collection | Call number | Status | Date due | Barcode | Item holds | |
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Online Document
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CIAT Library Web | Electronic Document | 35334 (Browse shelf(Opens below)) | Not For Loan (Restricted Access) | ||||
Books
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CIAT Library CIAT Publications | CIAT Publications | 35334 (Browse shelf(Opens below)) | Available |
Se describen los metodos de aislamiento y cultivo de protoplastos de yuca y Stylosanthes; la aplicacion de estos metodos requiere la regeneracion rutinaria de plantas a partir de protoplastos aislados. En yuca, el aislamiento de protoplastos a partir de hojas y apices de vastagos cultivados in vitro y de embriones somaticos, diferenciados a partir de segmentos foliares, fue posible mediante una mezcla enzimatica de celulasa Onozuka, hemicelulasa y Pectolyasa. Los protoplastos aislados se purificaron por filtracion y se cultivaron en medio liquido; regeneraron pared celular a los 1-2 dias de cultivo y se dividieron a los 3-4 dias. La frecuencia de division observada fue de 10-20 por ciento en cultivos de protoplastos de apices y de mesofilo foliar. En los cultivos originados a partir de embriones somaticos, esta frecuencia fue menor al 5 por ciento. En 3-4 semanas, los protoplastos en division formaron colonias. Las colonias plaqueadas formaron callos en varios medios de cultivo. Se han aislado y cultivado protoplastos de 10 var. de yuca. (CIAT)
Methods of isolation and culture of cassava and Stylosanthes protoplasts are described. The application of these methods requires the routine regeneration of plants from isolated protoplasts. In cassava, protoplast isolation from leaves and shoot tips of plants cultured in vitro and from somatic embryos, differentiated from leaf segments, was possible with an enzyme mixture consisting of Onozuka cellulase, hemicellulase, and Pectolyase. Isolated protoplasts were purified by filtration and cultured in liquid medium; they regenerated cell wall after 1-2 days of culture and presented cell division after 3-4 days. Division frequency observed was 10- 20 percent in protoplast cultures from leaf mesophyll and shoot tips. In cultures of somatic embryos, this frequency was less than 5 percent. Within 3-4 wk., dividing protoplasts formed colonies. The plated colonies formed callus in several culture media. Protoplasts from 10 cassava var. have been isolated and cultured. (CIAT)