Stability of cassava plants at the DNA level after retrieval from 10 years of in vitro storage
Material type:
ArticleLanguage: English Description: 90(3):307-313Subject(s): - Manihot esculenta
- Germplasm conservation
- Biological preservation
- Genetic stability
- Genetic markers
- Manihot esculenta
- Conservación del germoplasma
- Conservación biológica
- Estabilidad genética
- Marcadores genéticos
- Cassava
- Yuca
- CIAT Autor
- RJ
- Electronic documents
- Documentos electrónicos
- Genética vegetal y fitomejoramiento
- Thompson
- Propagación de plantas
- Plant genetics and breeding
- Plant propagation
- Articles in Refereed Journals
| Item type | Current library | Collection | Call number | Copy number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|---|
Online Document
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CIAT Library Web | Electronic Document | Not For Loan (Restricted Access) | ||||||
Journal Article
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CIAT Library Journal Collection | Journal Collection | c.1 | Not For Loan (Restricted Access) |
Cassava (Manihot esculenta Crantz) germplasm collections are conventionally maintained by continuous vegetative propagation in the field. Tissue culture techniques provide a more convenient way to conserve germplasm. The cassava in vitro gene bank held in trust at CIAT comprises nearly 6000 accessions. A study was carried out to determine whether any DNA rearrangements resulting from in vitro storage under slow growth could be detected by molecular analysis in retrieved plants. RFLPs with homologous probes, RAPDs with twenty primers and DNA fingerprinting with M13 probe were tested to detect variation at DNA level in cassava plants after ten-years in vitro storage. The molecular marker data obtained in this study supports the stability of the cassava germplasm under the in vitro storage conditions described in this work eng