000			02999naa a22004697a 4500
001			39649
003			CO-PlCIA
005			20130510154429.0
040			_aCO-PlCIA _cCO-PlCIA
041			_aeng
050			_a41174
245			_aInduction of chalcone isomerase gene expression in plant cells in response to fungal cell wall components
100			_aMEHDY, M.C.
700			_aLAMB, C.J.
008			120502t1987 000 0seng d
300			_ap. 425-435
520			_aPlants respond to irradiation by producing protective anthocyanin pigments and to infection by producing phytoalexin antibiotics. Synthesis of anthocyanins and some flavonoid pigments is also developmentally regulated and tissue-specific in leaves and flowers. Chalcone isomerase (CHI) catalyzes the formation of an intermediate common to both flavonoid pigment and isoflavonoid phytoalexin biosynthetic pathways and elevated CHI enzyme activity is correlated with the accumulation of these compounds in many plants. To determine the molecular basis for enzyme induction, we constructed a lambda gt 11 cDNA library prepared from mRNA from Phaseolus vulgaris cell cultures treated with a fungal cell wall preparation (elicitor). Two positive clones were obtained by screening 10(5) recombinants with an antiserum that had been raised against purified CHI. The identity of the cloned sequence was confirmed by showing that the largest clone (CHI 1) encoded polypeptides which were precipitated by the antiserum. Blot hybridization of RNA from elicitor treated cell cultures with labeled CHI l showed substantial accumulation of a 0.95 kb RNA within 3-4 h. The kinetics of CHI mRNA accumulation are similar to those of phenylalanine ammonia-lyase and chalcone synthase mRNAs, which encode other enzymes common to both pathways. CHI l hybridized to single fragments on genomic DNA blots suggesting a single gene. The structure and expression of the gene is being investigated to elucidate the regulatory sequences conferring developmental and environmental inducibility. (AS) _9eng
650			_aPhaseolus vulgaris _9eng
650			_aColletotrichum lindemuthianum _9eng
650			_aDISEASE PHYSIOLOGY AND BIOCHEMISTRY _9eng
650			_aTissue culture _9eng
650			_aGenes _9eng
650			_aDNA _9eng
650			_aRNA _9eng
650			_aEnzymes _9eng
650			_aPhaseolus vulgaris _9spa
650			_aColletotrichum lindemuthianum _9spa
650			_aFISIOLOGIA Y BIOQUIMICA DE LAS ENFERMEDADES _9spa
650			_aCultivo de tejidos _9spa
650			_aGenes _9spa
650			_aADN _9spa
650			_aARN _9spa
650			_aEnzimas _9spa
653			_aFrijol _9spa
653			_aBeans _9spa
653			_aBook chapters _9spa
653			_aCapítulos de libros _9spa
773			_tIn: Firtel, R.A.; Davidson, E.H. (eds.). Molecular approaches to developmental biology _dNew York, NY, USA, Alan R. Liss, 1987
998			_aFRIJOL
653			_aBook chapters _9eng
942			_2lcc _cBKC
999			_c29003 _d29003