| 000 | 02454nab a22004577a 4500 | ||
|---|---|---|---|
| 001 | 39649 | ||
| 003 | CO-PlCIA | ||
| 005 | 20130510171708.0 | ||
| 040 |
_aCO-PlCIA _cCO-PlCIA |
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| 041 | _aeng | ||
| 050 | _a60097 | ||
| 245 | _aAssessment of genetic diversity among strains of Xanthomonas campestris pv. manihotis | ||
| 100 | _aVerdier, Valérie | ||
| 700 | _aDongo, P. | ||
| 700 | _aBoher, Bernard | ||
| 008 | 120504t1993 000 0seng d | ||
| 300 | _a139:2591-2601 | ||
| 520 |
_aThree-hundred and twenty-six strains of Xanthomonas campestris pv. manihotis from 22 countries were studied to detect and assess genetic and evolutionary relationships within the pathovar. A range of techniques was used for this study including restriction fragment length polymorphism (RFLP) analysis. The probes used for the RFLP analysis were 16 plus 23S rRNA genes from Escherichia coli and three restriction fragments from the chromosomal and plasmid DNA of X. campestris pv. manihotis. Analysis of the rRNA probe data showed five RFLP groups whilst the other three probes were used to further sub-divide these groupings. Genetic variability of X. campestris pv. manihotis was pronounced in strains from South America where the host plant originated but was limited in strains from other regions. The results obtained confirm the hypothesis that the pathogen has been introduced only recently to Africa and suggests that African strains have not as yet diversified significantly at the chromosomal level. Our results indicate that rRNA and DNA probes are useful tools for epidemiological studies and in following the genetic evolution of strains. _9eng |
||
| 650 |
_aManihot esculenta _9eng |
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| 650 |
_aXanthomonas campestris _9eng |
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| 650 |
_aGenetic polymorphism _9eng |
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| 650 |
_aPathogenicity _9eng |
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| 650 |
_aManihot esculenta _9spa |
||
| 650 |
_aXanthomonas campestris _9spa |
||
| 650 |
_aPolimorfismo genético _9spa |
||
| 650 |
_aPatogenicidad _9spa |
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| 653 |
_aCassava _9spa |
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| 653 |
_aYuca _9spa |
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| 653 |
_aJournal articles _9spa |
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| 653 |
_aArtículos en revistas _9spa |
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| 653 |
_aRFLP _9spa |
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| 653 |
_aCIAT MENCION _9spa |
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| 653 |
_aEnfermedades de las plantas _9spa |
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| 653 |
_aRFLP _9eng |
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| 653 |
_aPlant diseases _9eng |
||
| 773 |
_tJournal of General Microbiology (United Kingdom) _d1993 |
||
| 998 | _aCATAL | ||
| 998 | _aYUCA | ||
| 653 |
_aJournal article _9eng |
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| 942 |
_2lcc _cJA |
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| 999 |
_c66988 _d66988 |
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