000			03529nab a22004937a 4500
001			39649
003			CO-PlCIA
005			20130510184311.0
040			_aCO-PlCIA _cCO-PlCIA
041			_aeng
050			_a33179
245			_a31P NMR study of elicitor treated Phaseolus vulgaris cell suspension cultures
100			_aOJALVO, I.
700			_aROKEM, J.S.
700			_aNAVON, G.
700			_aGOLDBERG, I.
008			120504t1987 000 0seng d
300			_a85(3):716-719
520			_aLa adicion de un desencadenante (glucano) a cultivos en suspension de celulas de Phaseolus vulgaris aumento la formacion de la fitoalexina faseolina. Se estudiaron el pH intracelular y las concn. de fosfato mediante espectroscopia de resonancia magnetica nuclear de (31)P en celulas tratadas con desencadenante, que se airearon durante la medicion de la resonancia magnetica nuclear. El pH de la vacuola, y en menor grado el pH del citoplasma, se afectaron 10 min despues de la adicion del desencadenante; se observo un descenso del pH de 5.3 a 4.8 en la vacuola y de 7.46 a 7.28 en el citoplasma. La proporcion entre la cantidad de Pi de la vacuola y la del citoplasma tambien cambio en los 10 min siguientes a la adicion del desencadenante. La senal para ATP (beta-ATP) fue inferior despues de la adicion del desencadenante y aumento de nuevo 23 h despues de la misma. Cuarenta y ocho h despues de la adicion del desencadenante, los pH vacuolar y citoplasmatico regresaron a aprox. sus valores iniciales. El cambio subito en pH vacuolar y citoplasmatico puede causar el cambio de metabolismo que ocurre en las celulas de P. vulgaris tratadas con desencadenante. (RA-CIAT) _9spa
520			_aThe addition of an elicitor (glucan) to Phaseolus vulgaris cell suspension cultures increased the formation of the phytoalexin phaseollin. Intracellular pH and phosphate concn. were studied with (31)P nuclear magnetic resonance spectroscopy on elicitor-treated cells which were aerated during the nuclear magnetic resonance measurement. The pH of the vacuole and to a lesser extent the pH of the cytoplasm were affected 10 min after elicitor addition; a decrease in pH from 5.3 to 4.8 was noted in the vacuole and from 7.46 to 7.28 in the cytoplasm. The ratio between the amount of Pi in the vacuole and that in the cytoplasm also changed within 10 min after elicitor addition. The signal for ATP (beta-ATP) was low after elicitor addition and high again 23 h after elicitation. Forty-eight h after elicitor addition, the vacuolar and cytoplasmic pH had almost returned to their initial values. The rapid change in vacuolar and cytoplasmic pH may cause the change of metabolism that occurs in elicitor-treated P. vulgaris cells. (AS) _9eng
650			_aPhaseolus vulgaris _9eng
650			_aAnalysis _9eng
650			_aCytology _9eng
650			_aIsrael _9eng
650			_aPHASEOLIN _9eng
650			_aAsia _9eng
650			_aPhytoalexins _9eng
650			_aPhaseolus vulgaris _9spa
650			_aFASEOLINA _9spa
650			_aANALISIS _9spa
650			_aCitologia _9spa
650			_aIsrael _9spa
653			_aJournal articles _9spa
653			_aArtículos en revistas _9spa
653			_aFrijol _9spa
653			_aBeans _9spa
653			_aElectronic documents _9spa
653			_aDocumentos electrónicos _9spa
856			_uhttp://www.plantphysiol.org/cgi/reprint/85/3/716
773			_tPlant Physiology _d1987
998			_aFRIJOL
653			_aJournal article _9eng
942			_2lcc _cJA
999			_c85548 _d85548