Almacenamiento del polen de yuca (Manihot esculenta Crantz) por medio de liofilizacion y varios regimenes de humedad y temperatura
ORREGO A., J.I.
Almacenamiento del polen de yuca (Manihot esculenta Crantz) por medio de liofilizacion y varios regimenes de humedad y temperatura - 34(1):21-25
Se realizaron ensayos de germinacion in vitro del polen de yuca var. MBRA 20 y MCOL 1413. Los medios contenian concn. de sacarosa de 0-70 por ciento y microelementos (150 ppm H3BO3 + 450 ppm Ca(NO3)2 + 150 ppm MgSO4 + 150 ppm KNO3). En ninguno de los medios germino el polen. La conservacion del polen de yuca var. MCOL 1413 se intento por diversos tratamientos (sin secar-control, secado con gel de silice durante 24 y 48 h, liofilizado 4 y 8 h). El polen luego se almaceno a 23.5, 8 y -12 grados centigrados durante 2 y 4 semanas. En los tratamientos que hubo produccion de semilla, ninguna resulto viable. El metodo de tincion del polen con acetocarmin al 1 por ciento no fue efectivo para determinar la viabilidad del polen almacenado pero si la del polen fresco. (RA) Trials were carried out on in vitro germination of pollen of cassava var. MBRA 20 and MCOL 1413. The media included sucrose concn. from 0 to 70 percent, with addition of microelements (150 ppm H3BO3 + 450 ppm Ca(NO3)2 + 150 ppm MgSO4 + 150 ppm KNO3). No germination was observed in any of the media utilized. Conservation of pollen of MCOL 1413 was attempted by various treatments, (no drying-control, drying over silica gel 24 and 48 h, freeze-dried 4 and 8 h), and later stored at 23.5, 8, and -12 degrees Celsius during 2 and 4 wk. For all treatments in which seed was produced after pollen storage, none of the seed was viable. The method of pollen staining by 1 percent acetocarmine as a test of pollen viability was effective for fresh pollen, but not for stored pollen. (AS)
Manihot esculenta
Colombia
Cultivars
Culture media
Germination
Pollen
POLLINATION
Storage
Temperature
ANTHERS
STAMENS
Flowers
Inflorescences
Plant anatomy
LABORATORY EXPERIMENTS
Research
Developmental stages
PLANT REPRODUCTION
South America
Colombia
América del Sur
RJ Cassava Yuca CIAT Autor Articles in Refereed Journals
25733
Almacenamiento del polen de yuca (Manihot esculenta Crantz) por medio de liofilizacion y varios regimenes de humedad y temperatura - 34(1):21-25
Se realizaron ensayos de germinacion in vitro del polen de yuca var. MBRA 20 y MCOL 1413. Los medios contenian concn. de sacarosa de 0-70 por ciento y microelementos (150 ppm H3BO3 + 450 ppm Ca(NO3)2 + 150 ppm MgSO4 + 150 ppm KNO3). En ninguno de los medios germino el polen. La conservacion del polen de yuca var. MCOL 1413 se intento por diversos tratamientos (sin secar-control, secado con gel de silice durante 24 y 48 h, liofilizado 4 y 8 h). El polen luego se almaceno a 23.5, 8 y -12 grados centigrados durante 2 y 4 semanas. En los tratamientos que hubo produccion de semilla, ninguna resulto viable. El metodo de tincion del polen con acetocarmin al 1 por ciento no fue efectivo para determinar la viabilidad del polen almacenado pero si la del polen fresco. (RA) Trials were carried out on in vitro germination of pollen of cassava var. MBRA 20 and MCOL 1413. The media included sucrose concn. from 0 to 70 percent, with addition of microelements (150 ppm H3BO3 + 450 ppm Ca(NO3)2 + 150 ppm MgSO4 + 150 ppm KNO3). No germination was observed in any of the media utilized. Conservation of pollen of MCOL 1413 was attempted by various treatments, (no drying-control, drying over silica gel 24 and 48 h, freeze-dried 4 and 8 h), and later stored at 23.5, 8, and -12 degrees Celsius during 2 and 4 wk. For all treatments in which seed was produced after pollen storage, none of the seed was viable. The method of pollen staining by 1 percent acetocarmine as a test of pollen viability was effective for fresh pollen, but not for stored pollen. (AS)
Manihot esculenta
Colombia
Cultivars
Culture media
Germination
Pollen
POLLINATION
Storage
Temperature
ANTHERS
STAMENS
Flowers
Inflorescences
Plant anatomy
LABORATORY EXPERIMENTS
Research
Developmental stages
PLANT REPRODUCTION
South America
Colombia
América del Sur
RJ Cassava Yuca CIAT Autor Articles in Refereed Journals
25733