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Almacenamiento del polen de yuca (Manihot esculenta Crantz) por medio de liofilizacion y varios regimenes de humedad y temperatura

By: Contributor(s): Material type: ArticleArticleLanguage: Spanish Description: 34(1):21-25Subject(s): LOC classification:
  • 25733
Online resources: In: Acta Agronomica (Colombia)Summary: Se realizaron ensayos de germinacion in vitro del polen de yuca var. MBRA 20 y MCOL 1413. Los medios contenian concn. de sacarosa de 0-70 por ciento y microelementos (150 ppm H3BO3 + 450 ppm Ca(NO3)2 + 150 ppm MgSO4 + 150 ppm KNO3). En ninguno de los medios germino el polen. La conservacion del polen de yuca var. MCOL 1413 se intento por diversos tratamientos (sin secar-control, secado con gel de silice durante 24 y 48 h, liofilizado 4 y 8 h). El polen luego se almaceno a 23.5, 8 y -12 grados centigrados durante 2 y 4 semanas. En los tratamientos que hubo produccion de semilla, ninguna resulto viable. El metodo de tincion del polen con acetocarmin al 1 por ciento no fue efectivo para determinar la viabilidad del polen almacenado pero si la del polen fresco. (RA)Summary: Trials were carried out on in vitro germination of pollen of cassava var. MBRA 20 and MCOL 1413. The media included sucrose concn. from 0 to 70 percent, with addition of microelements (150 ppm H3BO3 + 450 ppm Ca(NO3)2 + 150 ppm MgSO4 + 150 ppm KNO3). No germination was observed in any of the media utilized. Conservation of pollen of MCOL 1413 was attempted by various treatments, (no drying-control, drying over silica gel 24 and 48 h, freeze-dried 4 and 8 h), and later stored at 23.5, 8, and -12 degrees Celsius during 2 and 4 wk. For all treatments in which seed was produced after pollen storage, none of the seed was viable. The method of pollen staining by 1 percent acetocarmine as a test of pollen viability was effective for fresh pollen, but not for stored pollen. (AS)
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Journal Article Journal Article CIAT Library Document collection CINFOS Document Collection CINFOS 25733 (Browse shelf(Opens below)) c.1 Short Loan 100055611
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Se realizaron ensayos de germinacion in vitro del polen de yuca var. MBRA 20 y MCOL 1413. Los medios contenian concn. de sacarosa de 0-70 por ciento y microelementos (150 ppm H3BO3 + 450 ppm Ca(NO3)2 + 150 ppm MgSO4 + 150 ppm KNO3). En ninguno de los medios germino el polen. La conservacion del polen de yuca var. MCOL 1413 se intento por diversos tratamientos (sin secar-control, secado con gel de silice durante 24 y 48 h, liofilizado 4 y 8 h). El polen luego se almaceno a 23.5, 8 y -12 grados centigrados durante 2 y 4 semanas. En los tratamientos que hubo produccion de semilla, ninguna resulto viable. El metodo de tincion del polen con acetocarmin al 1 por ciento no fue efectivo para determinar la viabilidad del polen almacenado pero si la del polen fresco. (RA)

Trials were carried out on in vitro germination of pollen of cassava var. MBRA 20 and MCOL 1413. The media included sucrose concn. from 0 to 70 percent, with addition of microelements (150 ppm H3BO3 + 450 ppm Ca(NO3)2 + 150 ppm MgSO4 + 150 ppm KNO3). No germination was observed in any of the media utilized. Conservation of pollen of MCOL 1413 was attempted by various treatments, (no drying-control, drying over silica gel 24 and 48 h, freeze-dried 4 and 8 h), and later stored at 23.5, 8, and -12 degrees Celsius during 2 and 4 wk. For all treatments in which seed was produced after pollen storage, none of the seed was viable. The method of pollen staining by 1 percent acetocarmine as a test of pollen viability was effective for fresh pollen, but not for stored pollen. (AS)

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