Cholinergic and non-cholinergic projections from the rat basal forebrain revealed by combined choline acetyltransferase and Phaseolus vulgaris leucoagglutinin immunohistochemistry

By:

WOOLF, N.J

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Material type: Article

ArticleLanguage: English Description: 66(3):281-286Subject(s): LOC classification:

28941

In: Neuroscience LettersSummary: Se descibe un metodo de fluorescencia de 2 colores para demostrar inmunohistoquimicamente la lectina leucoaglutinina de Phaseolus vulgaris (PHAL, marcada con isotiocianato de fluoresceina) transportado anterogradamente y la acetiltransferasa de colina (ChAT, marcada con rodamina) en la misma seccion del cerebro de la rata. La aplicacion de este metodo en el estudio de neuronas de proyeccion en los miembros vertical y horizontal de la banda diagonal, la substantia innominada y el oliva bulbar revelo que las vias tanto colinergicas como no colinergicas siguieron trayectorias similares hacia sus objetivos. (RA (extracto)-CIAT)Summary: A 2-color fluorescence method is described for demonstrating immunohistochemically the anterogradely transported plant lectin Phaseolus vulgaris leucoagglutinin (PHAL, fluorescein isothiocyanate label) and choline acetyltransferase (ChAT, rhodamine label) on the same rat brain section. Application of this method to the study of projection neurons in the vertical and horizontal limbs of the diagonal band, the substantia innominata, and nucleus basalis revealed that both cholinergic and noncholinergic pathways followed similar trajectories to their targets. (AS (extract))

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Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode	Item holds
Journal Article	CIAT Library Document collection CINFOS	Document Collection CINFOS	28941 (Browse shelf(Opens below))	c.1	Short Loan		100065842

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Previous	28938 The effect of solar drying and heating on the hardness of Phaseolus beans during storage	28939 Intervention des racines dans la sensibilite ou la tolerance a NaCl de plantes cultivees	28940 Studies on persistence pattern and safety evaluation of synthetic pyrethroids on French bean	28941 Cholinergic and non-cholinergic projections from the rat basal forebrain revealed by combined choline acetyltransferase and Phaseolus vulgaris leucoagglutinin immunohistochemistry	28942 Purification and partial characterization of two alpha-amylase inhibitors from black bean (Phaseolus vulgaris)	28943 Metabolism of trypsin-inhibitory proteins in the germinating seeds of kidney bean (Phaseolus vulgaris)	28944 Metodo da fibra detergente neutro modificado para amostras ricas em amido	Next

Se descibe un metodo de fluorescencia de 2 colores para demostrar inmunohistoquimicamente la lectina leucoaglutinina de Phaseolus vulgaris (PHAL, marcada con isotiocianato de fluoresceina) transportado anterogradamente y la acetiltransferasa de colina (ChAT, marcada con rodamina) en la misma seccion del cerebro de la rata. La aplicacion de este metodo en el estudio de neuronas de proyeccion en los miembros vertical y horizontal de la banda diagonal, la substantia innominada y el oliva bulbar revelo que las vias tanto colinergicas como no colinergicas siguieron trayectorias similares hacia sus objetivos. (RA (extracto)-CIAT) spa

A 2-color fluorescence method is described for demonstrating immunohistochemically the anterogradely transported plant lectin Phaseolus vulgaris leucoagglutinin (PHAL, fluorescein isothiocyanate label) and choline acetyltransferase (ChAT, rhodamine label) on the same rat brain section. Application of this method to the study of projection neurons in the vertical and horizontal limbs of the diagonal band, the substantia innominata, and nucleus basalis revealed that both cholinergic and noncholinergic pathways followed similar trajectories to their targets. (AS (extract)) eng